目的 研究野百合Crotalaria sessiliflora全草的异黄酮类化学成分。方法 利用硅胶柱、葡聚糖凝胶柱、MCI等柱色谱技术分离纯化,根据核磁共振波谱鉴定化合物结构。结果 从野百合全草75%乙醇中分离得到21个异黄酮类化合物,分别鉴定为6,7,2'-三甲氧基-4'-羟基异黄酮(1)、7,4'-二羟基-5-甲氧基异黄酮(2)、大豆苷元(3)、2'-羟基染料木素(4)、染料木素(5)、3'-羟基大豆苷元(6)、genistein 7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(7)、染料木苷(8)、光果甘草酮(9)、alpinumisoflavone(10)、barbacarpan(11)、3'-羟基染料木素(12)、4',7-二甲氧基-5-羟基异黄酮(13)、红车轴草素(14)、8-甲雷杜辛(15)、黄甘草异黄酮A(16)、樱黄素(17)、芒柄花素(18)、2'-methylalpinumisoflavone(19)、hydroxyalpinumisoflavone(20)、desmoxyphllin A(21)。结论 化合物1为新化合物,命名为野百合素A;化合物2～3、6～21是首次从该植物中分离得到。

Objective To investigate the isoflavones constituents from the whole plant of Crotalaria sessiliflora. Methods The compounds were isolated by column chromatography and their structures were identified by the spectral data. Results Twenty-one isflavones were isolated from 75% ethanol extract in the whole plant of C. sessiliflora and identified as 6,7,2'-trimethoxy-4'-hydroxyl isoflavone (1), 7,4'-dihydroxy-5-methoxy isoflavone (2), daidzein (3), 2'-hydroxyl genistein (4), genistein (5), 3'-hydroxyl daidzein (6), genistein 7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (7), genistein 7-O-glucoside (8), glabrone (9), alpinumisoflavone (10), barbacarpan (11), 3'-hydroxyl genistein (12), 4',7-dimethoxy-5-hydroxyl isoflavone (13), pratensein (14), 8-O-methylreyusi (15), eurycarpin A (16), prunetin (17), formononetin (18), 2'-methylalpinumisoflavone (19), hydroxyalpinumisoflavone (20), and desmoxyphllin A (21). Conclusion Compound 1 is a new compound named sessiliflorin A, compounds 2—3, and 6—21 are isolated from the plant for the first time.

国家自然科学基金面上项目(21272159);上海市自然科学基金项目(12ZR1431600)