目的 对傣药竹叶兰Arundina graminifolia全株的化学成分进行研究,以期发现新的活性化合物。方法 运用硅胶、凝胶、MCI-GEL树脂及PR-HPLC等多种色谱技术进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构,并采用半叶法检测化合物的抗烟草花叶病毒活性。结果 从竹叶兰氯仿-甲醇(1:1)提取物中分离得到4个苯丙素类化合物,分别鉴定为6-(3-羟基丙酰)-5-甲氧基-异苯并呋喃-1(3H)-酮(1)、香豆酸(2)、ω-羟基化愈创木丙酮(3)、3-甲氧基-4-羟基-苯丙醇(4)。结论 化合物1为新化合物,命名为竹叶兰苯丙素,化合物1表现出明显的抗烟草花叶病毒活性,其相对抑制率为22.6%。

Objective For the purpose of finding new bioactive agents from ethnic medicines, the chemical study on Dai Medicine Arundina graminifolia was carried out. Methods The chemical constituents in the whole plant of A. graminifolia were isolated by silica gel, MCI-GEL resin, Sephadex LH-20 column chromatography, and high performance liquid chromatography (HPLC) method. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. The inhibitory activities of compound 1 against tobaco mosaic virus (TMV) replication were tested using the half-leaf method. Results Four phenylpropanoids, 6-(3-hydroxypropanoyl)-5-hydroxymethyl-isobenzofuran-1(3H)-one (1), coumaric acid (2), ω-hydroxypropioguaiacone (3), and 3-methoxy-4-hydroxy-propyl alcohol (4) were isolated from the whole plant of A. graminifolia. Conclusion Compound 1 is isolated from the whole plant of A. graminifolia for the first time. Compound 1 is a new compound named Arundina phenylpropanoid. It is tested for its anti-TMV activities. The results show that compound 1 exhibits the potential anti-TMV activities with the inhibitory rate of 22.6%.

国家自然科学基金资助项目(21362044,21032085);云南省高校科技创新团队资助项目(IRTSTYN2014-11)