目的 建立不同产地何首乌Polygonum multiflorum的HPLC指纹图谱。方法 采用HPLC法建立不同产地16批何首乌药材的指纹图谱,色谱条件为Extend C₁₈色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.1%甲酸溶液为流动相,梯度洗脱,体积流量1.0 mL/min,检测波长280 nm,通过Matlab软件对共有峰面积进行主成分分析,同时对16批何首乌中的二苯乙烯苷进行测定。结果 16批何首乌的相似度在0.936～0.998,说明不同产地何首乌样品质量稳定。同时确定了18个共有峰,并通过HPLC/Q-TOF/MS对何首乌共有峰进行定性分析。主成分分析结果与相似度分析结果一致,同时二苯乙烯苷量与主成分分析结果一致。结论 该方法简便、可靠,可为何首乌药材的质量控制提供指导和依据。

Objective To establish an HPLC-fingerprint method for evaluating the quality of the root of Polygonum multiflorum from different habitats in China. Methods A separation was performed on an Agilent extend C₁₈ chromatographic column (250 mm × 4.6 mm, 5 μm) with gradient elution. The mobile phase consisted of acetonitrile-formic acid water solution (0.1%). And the mobile phase was at flow rate of 1.0 mL/min. The detection wavelength was at 280 nm. HPLC fingerprint chromatograms of 16 batches of crude material of the roots of P. multiflorum were analyzed. Chemometric methods including principle component analysis (PCA), and clustering analysis (CA) were used through the software of Matlab for common peaks. And the content of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside was determined by HPLC. Results The similarities of the 16 batches of samples were between 0.936 and 0.998. This showed that the quality of the roots of P. multiflorum from different habitats in China was stable. Eighteen common peaks were identified by HPLC/Q-TOF/MS. The results of FCA and CA were consistent with similarity evaluation. The content of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside was consistent with principle component loading factors. Conclusion The method is simple reliable, and it can provide a standard and guidance for the quality control of the roots of P. multiflorum.

国家“十二五”科技支撑计划项目(2011BAI13B04);2011江苏省中药资源产业化过程协同创新中心建设首批重点项目(ZDXM-1-3);贵州省中药现代化专项(黔科合中药字[2013]5059号)