目的 对淫羊藿Epimedium brevicornu中的化学成分进行研究。方法 采用D101大孔树脂柱色谱、硅胶柱色谱、Sephadex LH-20柱色谱和制备液相色谱等方法进行分离纯化,根据理化性质及波谱数据鉴定化合物的结构。结果 从淫羊藿总黄酮提取物中分离得到11个化合物,分别鉴定为5-羟基-2-异戊烯基苯酚-1-O-β-D-吡喃葡萄糖苷(1)、kaempferol-3-O- rhamnopranosyl-(1→2)-rhamnopyranoside(2)、3-O-α-L-rhamnosyl-7-O-β-D-glucosyl kaempferol(3)、sagittasine C(4)、山柰酚-3-O-[α-L-鼠李糖基 (1→6)-β-D-半乳糖苷]-7-O-α-L-鼠李糖苷(5)、槲皮素-3,7-二氧-α-L-双鼠李糖苷(6)、山柰酚-3,7-O-α-L-二鼠李糖苷(7)、rhamnocitrin-3-O-β-neohesperidoside(8)、山柰酚-3-O-α-L-鼠李糖-7-O-[α-L-鼠李糖基(1→2)-β-D-葡萄糖] 苷(9)、hexandraside E(10)和柔藿苷(11)。结论 其中化合物1、5为淫羊藿中首次分得,化合物2、3、4、6和9为淫羊藿属植物中首次分得。

Objective To study the chemical constituents of Epimedium brevicornu. Methods Compounds were repeatedly separated and purified with D101 macroporous resin column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography and preparative liquid chromatography. Their structures were identified by physico-chemical methods and spectral methods. Results Six xompunds were identified as 5-hydroxy-2-prenylphenol-1-O-β-D-glucopyranoside (1), kaempferol-3-O- rhamnopranosyl-(1→2)-rhamnopyranoside (2), 3-O-α-L-rhamnosyl-7-O-β-D-glucosyl kaempferol (3), sagittasine C (4), kaempferol-3- O-[α-L-rhamnopyra-nosyl-(1→6)-β-D-galactopyranoside]-7-O-α-L-rhamnopyranoside (5), 3,7-di-O-α-L-rhamnopyranosyl quercetin (6), Kaempferol-3,7-O-α-L-dirhamnoside (7), hamnocitrin-3-O-β-neohesperidoside (8), kaempferol-3-O-α-L-rhamnopyranoside-7- O-[α-L-rhamnopyranosyl (1→2)-β-D-glucopyranoside] (9), hexandraside E (10), and rouhuoside (11). Conclusion Compounds 1 and 5 are isolated from this plant for the first time. Compounds 2—4 and 6 are isolated from plants of this genus for the first time.

科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)