目的 研究地桃花Urena lobata的化学成分。方法 利用硅胶、Sephadex LH-20柱色谱及半制备液相色谱等方法分离纯化,根据理化性质及MS、NMR等光谱数据鉴定化合物的结构。结果 从地桃花醋酸乙酯提取部位分离得到14个化合物,分别鉴定为芹菜素-6-C-(6"-O-反式咖啡酰基)-β-D-吡喃葡萄糖苷(1)、银锻苷(2)、山柰酚-3-O-(6"-O-顺式对香豆酰基)-β-D-吡喃葡萄糖苷(3)、山柰酚-3-O-β-D-吡喃葡萄糖-(1→2)-β-D-吡喃半乳糖苷(4)、芦丁(5)、紫云英苷(6)、黄芩苷(7)、杨梅苷(8)、异槲皮苷(9)、黄芩素(10)、木犀草素(11)、芹菜素(12)、山柰酚(13)、槲皮素(14)。结论 化合物1为新化合物,命名为地桃花苷A;化合物3、4、7、8为首次从锦葵科植物中分离得到,化合物10为首次从肖梵天花属植物中分离得到。

Objective To study the chemical constituents of Urena lobata. Methods Compounds were isolated and purified using various column chromatographies such as silica gel, Sephadex LH-20, and prep HPLC. Their structures were identified by physicochemical properties and various spectroscopic experiments, including HRESIMS, ¹H-NMR, ¹³C-NMR, HSQC, and HMBC. Results Fourteen flavonoids were obtained from the ethyl acetate extract of U. lobata, including apigenin-6-C-(6"-O-trans- caffeoyl)-β-D-glucopyranoside (1), tiliroside (2), kaempferol-3-O-(6"-O-cis-p-coumaroyl)-β-D-glucopyranoside (3), kaempferol-3- O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside (4), rutin (5), astragalin (6), baicalin (7), myricetrin (8), isoquercitrin (9), baicalein (10), luteolin (11), apigenin (12), kaempferol (13), and quercetin (14). Conclusion Compound 1 is a new compound named urenalobside A, compounds 3, 4, 7, and 8 are firstly obtained from the family Malvaceae, and compound 10 is firstly isolated from genus Urena Linn.

国家工信部中药材扶持项目;国家药典委员会“2015年版《中国药典》科研课题资助项目”