目的 对四倍体金银花水提物的抗炎作用以及急性毒性进行评价。方法 以二倍体金银花为对照, 分别采用角叉菜胶诱导大鼠足肿胀实验、大鼠棉球肉芽肿实验对四倍体金银花水提物进行抗炎活性评价;采用Bliss法测定小鼠的半数致死量(LD₅₀)对其进行急性毒性评价。结果 四倍体金银花水提物具有明显的抗急性炎症作用, 与亲本二倍体金银花之间抗炎作用差异不具有统计学意义, 2个种质均不具有明显的抗亚急性炎症作用。四倍体和二倍体金银花水提物ig给药对小鼠的LD₅₀值分别为72.12、69.92 g/kg, 急性毒性差异不明显, 分别相当于人体(体质量60 kg)安全用量的412和400倍。结论 四倍体金银花水提物具有明显的抗急性炎症作用, 在一定剂量范围内使用较为安全。

Objective To evaluate the anti-inflammatory effects and acute toxicity of the aqueous extract form tetraploid Lonicerae Flos. Methods Compared with the diploid, the paw swelling of rats induced by carrageenan and cotton pellet granuloma tests were obtained to study the anti-inflammatory effects of the tetraploid Lonicerae Flos. A pretest was used to judge the possibility of LD₅₀ in the acute toxicity test. The experimental data are calculated LD₅₀ on mice by Bliss method to evaluate the acute toxicity of the diploid Lonicerae Flos. Results The tetraploid Lonicerae Flos had the significant resistance to acute inflammation, but there was not significance between the two ploidies. Neither the tetraploid nor the diploid had the obvious subacute inflammation resistance effects. The possibility of measuring LD₅₀ was definite through pre-test. The LD₅₀ of the aqueous extract from the two ploidies were 72.12 and 69.92 g/kg, respectively. However, there was no obvious difference in acute toxicity between the two ploidies. And the LD₅₀ of mice was equal to 412 and 400 times of 60 kg normal human's daily dried medicinal herb expenses respectively. Conclusion The tetraploid Lonicerae Flos has the significant anti-inflammation with less toxic and side effect. It could be used safely in a certain dose range.

国家自然科学基金资助项目(31370360)