目的 对甘草Glycyrrhiza uralensis 根及根茎的化学成分进行研究。方法 采用硅胶柱、ODS柱色谱、制备液相色谱等分离技术进行分离纯化, 利用UV、MS、1D-NMR、2D-NMR等波谱数据鉴定化合物结构。结果 从甘草正丁醇部分分离得到14个化合物, 分别鉴定为macedonoside E(1)、22β-乙酰基乌拉尔甘草皂苷C(2)、甘草酸(3)、乌拉尔甘草皂苷F(4)、甘草皂苷G₂(5)、22β-乙酰基甘草醛(6)、甘草酸甲酯(7)、甘草素(8)、柚皮素(9)、异甘草素(10)、芒柄花苷(11)、甘草苷(12)、异佛来心苷(13)、芹糖甘草苷(14)。结论 化合物1和2为新化合物。

Objective To study the chemical constituents from the roots and rhizomes of Glycyrrhiza uralensis. Methods The roots and rhizome of G. uralensis were extracted with 95% EtOH. Then the extract was partitioned with petroleum ether, ethyl acetate, and n-butanol successively. The n-butanol fraction was isolated by silica gel and ODS column chromatography and preparative HPLC. The structures of compounds were identified by spectroscopic methods (UV, MS, 1D-NMR, and 2D-NMR). Results Fourteen compounds were obtained from the n-butanol fraction. They were macedonoside E (1), 22β-acetyl-uralsaponin C (2), glycyrrhizic acid (3), uralsaponin F (4), licorice-saponin G₂ (5), 22β-acetoxyl-glycyrrhaldehyde (6), 3β-O-[β-D-(6-methyl)-glucuronopyranosyl-(1→2)-β-D-glucuronopyranosyl]-glycyrrhizic acid (7), liquiritigenin (8), naringenin (9), isoliquiritigenin (10), ononin (11), liquiritin (12), isoviolanthin (13), and liquiritin apioside (14). Conclusion Macedonoside E (1) and 22β-acetyl-uralsaponin C (2) are new compounds.

国家重点基础研究发展计划("973"计划)项目(2012CB518405)