目的 采用HPLC法测定并比较分析藤合欢中山柰苷的量。方法 采用甲醇超声处理方法提取藤合欢, HPLC法测定山柰苷的量, 色谱柱为Agilent Zorbax Eclipse Plus C₁₈ 柱 (250 mm×4.6 mm, 5 μm), 甲醇-0.01 mol/L磷酸二氢钾溶液-冰醋酸水溶液(40:60:1.5)等度洗脱, 体积流量1.0 mL/min, 检测波长254 nm, 柱温30 ℃。结果 线性范围在0.092 7～0.926 7 μg(r=0.999 8), 平均加样回收率为100.8%, RSD为1.1%(n=6)。结论 该方法简便、准确, 重复性好, 可用于藤合欢质量控制;不同产地的藤合欢样本质量存在差异, 贮藏时间越久, 其所含的黄酮类成分越少。

Objective To establish an HPLC method for the determination of kaempferitrin in Celastri Orbiculati Fructus and to compare the quality of samples from different habitats in northeast China. Methods The extracts were obtained by methanol ultrasonic method and kaempferitrin was determinated by HPLC. Chromatographic separation was achieved on an Agilent Zorbax Eclipse Plus C₁₈ column (250 mm × 4.6 mm, 5 μm) at 30 ℃ and the mobile phase was a mixture of methanol- 0.01 mol/L solution of potassium dihydrogenphosphate -glacial acetic acid (40:60:15). The flow rate was 1.0 mL/min and the detection wavelength was 254 nm. Results The linearity range was 0.092 7—0.926 7 μg (r = 0.999 8), average recovery was 100.8%, and RSD = 1.1% (n = 6). Conclusion This method is validated in terms of selectivity, linearity, precision, and accuracy. It can be used as the effective method for quality control. The determination results show that the longer the fruit sits followed by picking, the less contents it has.

国家药典会《中国药典》2015年版项目