目的 优选纳米有机金属框架(IRMOF-3)与精氨酸-甘氨酸-天冬氨酸(Arg-Gly-Asp)序列组成的短肽(RGD)偶联的最佳制备工艺。方法 通过RGD对IRMOF-3进行修饰得到RIR纳米粒, 并对得到的RIR纳米粒通过红外光谱(IR)、质谱(MS)和扫描电镜(SEM)等手段进行定性分析, 以RGD与IRMOF-3的质量比、反应时间、反应温度为考察对象, 以偶联率为评价指标, 寻找最佳工艺条件。结果 IR、MS和SEM显示RGD成功偶联到IRMOF-3表面, 并获得RIR最佳工艺为A₁B₂C₁, 即RGD与IRMOF-3的质量比为1:1、反应时间为4 h、反应温度为-20 ℃时, 可以得到最大偶联率的RIR纳米粒。结论 IR、MS、SEM可以强有力地说明RIR纳米粒的成功偶联, 且正交试验优化出的RGD与IRMOF-3偶联的最优工艺, 值得推广使用。

Objective To optimize nano metal organic framework IRMOF-3 and RGD peptide by connecting the optimum preparation process. Methods To modify IRMOF-3 by RGD to obtainn RIR nanoparticles, and to qualitatively analyze the obtained RIR nanoparticles by infrared spectroscopy (IR), mass spectrometry (MS), scanning electron microscopy (SEM), etc. To find the optimum process conditions by taking IRMOF-3 and RGD mass ratio, reaction time at low temperature, low reaction temperature as the object of study and the coupling rate as the evaluation index. Results The analyses by IR, MS, and SEM showed that RGD was successfully coupled to the IRMOF-3 surface, and the best process for the obtained RIR was A₁B₂C₁. The mass ratio of folic acid and carrier is 1:1, the reaction time is 4 h, and the reaction temperature is -20 ℃, the RIR with maximum coupling rate of could be obtained. Conclusion IR, MS, and SEM could strongly suggest that RIR nanoparticles could be successfully coupled, the optimal process of RGD and IRMOF-3 coupling by orthogonal test is worthy to be popularized.

国家自然科学基金——中药纳米缓释有机金属框架载药体系的构建(2014bs06)