目的 考察石榴皮Granati Pericarpium超微粉的制备工艺以及石榴皮超微粉体内抗氧化能力。方法 通过单因素试验与响应面分析法对石榴皮超微粉的制备工艺进行优化, 选取最优条件下制备的石榴皮超微粉、石榴皮粗粉、水溶性维生素E(VE)和生理盐水(空白对照)进行大鼠体内抗氧化研究。结果 石榴皮超微粉制备工艺的最佳条件为粉碎时间25 min、粉碎温度-17 ℃、进料量198 g, 在该条件下验证石榴皮超微粉粒径可达到7.68 μm, 接近于预测值7.96 μm。优化得到的回归模型具有良好的预测能力。石榴皮超微粉组和VE组相对于石榴皮粗粉组和空白对照组可以显著提高大鼠的血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活力, 并有效减少血清中丙二醛(MDA)的量。结论 石榴皮超微粉具有较强的抵御膜脂质过氧化和清除血清中自由基的能力, 这表明石榴皮超微粉中的活性成分较粗粉可以更快、更好地得到释放, 从而表现出更强的体内抗氧化活性。

Objective Processing technology and antioxidant activity in vivo of pomegranate superfine powder were investigated. Methods Processing technology of superfine powder of Granati Pericarpium was optimized using single-factor tests and response surface methodology (RSM). The antioxidant effect in vivo of superfine powder of Granati Pericarpium with different particle, vitamin E, and normal saline were studied. Results The optimally grinding conditions for Granati Pericarpium were grinding time of 25 min, grinding temperature of -17 ℃, and input quantity of 198 g. Under the optimally grinding conditions, the minimum particle size of superfine powder of Granati Pericarpium was 7.68 μm which was close to the predicted value of 7.96 μm. Therefore, the established regression model has good prediction capability. Experimental results show that the superfine powder and vitamin E groups compared with the coarse powder and blank groups could significantly improve the activity of SOD, CAT, and GSH-Px, and reduce the content of MDA in serum of mice. Conclusion The ability of superfine powder group on the protection of membrane lipid peroxidation and scavenge free radical is quite superior. It indicates that the active ingredients in the superfine powder of Granati Pericarpium could be dissolved better and more quickly in solvent than those in coarse powder. Therefore, the superfine powder of Granati Pericarpium has the better anti-oxidant effect in vivo.

国家科技支撑计划项目(2012BAD21B05)