目的 研究九味肝泰胶囊对高脂饮食诱导的大鼠非酒精性脂肪性肝病(NAFLD)的治疗作用和可能机制。方法 采用高脂饮食诱导大鼠NAFLD动物模型。设对照组,模型组,水飞蓟素阳性对照组(0.05 g/kg),九味肝泰胶囊高、中、低剂量(1.80、0.90、0.45 g/kg)组。除对照组给予普通饲料饲养外,其他各组以高脂饲料喂养,饲养10周。从第5周开始,4个给药组分别ig给予相应药物,共给药6周。观察和检测大鼠体质量、肝湿质量、肝脏指数和肝脏形态学变化;检测血清中的高密度脂蛋白-胆固醇(HDL-C)、游离脂肪酸(FFA)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平;检测肝脏组织中总胆固醇(TC)、三酰甘油(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果 与模型组比较,九味肝泰胶囊高、中剂量组能显著降低NAFLD大鼠血清中的FFA、AST、ALT水平(P < 0.05),升高HDL-C水平(P < 0.05);显著降低NAFLD大鼠肝脏组织中的TC、TG、MDA水平(P < 0.05),升高SOD的活性(P < 0.05);NAFLD大鼠的肝组织病理学形态均得到不同程度改善,大鼠肝组织脂肪变程度减轻。结论 九味肝泰胶囊对NAFLD大鼠具有调节脂质和保护肝脏的作用,其作用机制可能与减少脂质在肝脏的沉积、减轻过氧化损伤有关。

Objective To study the therapeutical effect of Jiuwei Gantai Capsule (JWGT) on nonalcoholic fatty liver disease (NAFLD) of rats induced by high fat diet and its possible therapeutic mechanism. Methods The experimental rats were divided into six groups: normal group, model group, silymarin group (0.05 g/kg), JWGT high-, mid- and low-dose groups (1.80, 0.90, and 0.45 g/kg). Except the normal group, NAFLD rats were fed with high fat diet for 10 weeks, those other groups were ig given relevant medicine from the week 5 . All the rats were put to death after 10 weeks, the changes of body weight, liver weight, liver index, and hepatic histopathological morphology were observed. The high density lipoprotein cholesterol (HDL-C), free fatty acid (FFA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in serum were collected for the contents detection. Total choesterol (TC), triacylglycerol (TG), superoxide dismutase (SOD), and malondialdehyde (MDA) in hepatic tissue were detected, too. Results Compared with the model group, the content of FFA, AST, and ALT in serum of NAFLD rats were decreased (P < 0.05), while HDL-C was obviously improved (P < 0.05) in JWGT high- and mid-dose groups. The TC, TG, and MDA in the hepatic tissue were obviously decreased (P < 0.05), while the activity of SOD was significantly improved (P < 0.05). Meanwhile, the pathological images of hepatic tissue and classified graded method of steatosis were improved in NAFLD rats. Conclusion JWGT has the regulation of lipid and protection of liver for NAFLD rats. Its therapeutic mechanism is partly related to regulating lipid metabolism and anti-oxidative damage.