目的 研究优化大孔吸附树脂纯化益智总黄酮的最佳工艺。方法 以吸附率和解吸率为指标,利用静态吸附试验对15种大孔吸附树脂进行筛选,并通过与动态吸附相结合的方法,优选树脂的最佳分离纯化条件。结果 HP20大孔吸附树脂宜于FAOF的提纯,最佳纯化工艺为上样液黄酮质量浓度为1.518 mg/mL,上样体积流量为1.0 mL/min,上样量为50 mL,洗脱溶剂为30%乙醇,洗脱体积流量为1.0 mL/min,洗脱体积为6 BV,纯化后黄酮量高达51.68%。结论 HP20大孔吸附树脂能有效分离纯化益智中的总黄酮。

Objective To optimize the purification technology for flavone from Alpiniae Oxyphyllae Fructus (FAOF) with macroporous resins. Methods Static and dynamic adsorption-desorption were used to select the best one from 15 different type macroporous resins; With the content of flavone as index, the purification technology parameters of flavone were optimized. Results HP20 type resin showed the best purifying profile, its optimum technology conditions were as follows: sample injection concentration was 1.518 mg/mL, sample flow rate was 1.0 mL/min, quantity of adsorption was 50 mL, desorption rate was 1.0 mL/min, eluted by 6 BV 30% ethanol. Conclusion The flavone could be isolated from Alpiniae Oxyphyllae Fructus by HP20 macroporous resins.

国家自然科学基金项目—基于活性筛选和谱效表征的益智抗阿尔茨海默病药效物质基础研究(81403065);辽宁省自然科学基金—基于体内过程的中药镇静催眠活性组分筛选研究(2014020076);中药药效物质基础筛选与评价重点实验室(F13-287-1-00)