目的 研究铁皮石斛Dendrobium officinale的化学成分。方法 使用硅胶色谱法、反相硅胶色谱法及半制备HPLC等技术进行分离纯化,采用现代波谱技术鉴定化合物结构。结果 从铁皮石斛70%乙醇提取物中分离得到14个化合物,分别鉴定为苯甲醇-O-β-D-吡喃葡萄糖苷(1)、异鼠李素-3-O-α-L-鼠李糖基 (1→2)-β-D-吡喃葡萄糖苷(2)、conicaoside(3)、3,4,5-三甲氧基苯-1-O-β-D-吡喃葡萄糖苷(4)、2,6-二甲氧基-4-羟基苯酚-1-O-β-葡萄糖苷(5)、4-(3'-羟丙基)-2,6-二甲氧基苯酚- 3'-O-β-D-葡萄糖苷(6)、胸腺嘧啶脱氧核苷(7)、紫丁香苷(8)、4-烯丙基-2,6-二甲氧基苯基葡萄糖苷(9)、(+)-丁香脂素(10)、丁香脂素-4'-O-β-D-单葡糖苷(11)、柚皮素(12)、对羟基苯甲酸(13)、4,4'-二羟基-3,5-二甲氧基联苄(14)。结论 化合物1～6为首次从石斛属植物中发现,7～8为首次从本植物中发现。

Objective To study the chemical constituents of Dendrobium officinale in order to provide more scientific basis for its development and utilization of medicinal resources. Methods The compounds were isolated and purified by repeated column chromatography with silica gel and ODS column, and semi-preparative HPLC. Their structures were identified by spectroscopic data. Results Fourteen compounds were isolated from the 70% ethanol extract of D. officinale and identified as: benzyl-O-β-D- glucopyranoside (1), isorhamnetin-3-O-α-L-rhamnopyranosyl (1→2)-β-D-glycopyranoside (2), conicaoside (3), 3,4,5-trimethoxyphenyl- 1-β-D-glucopyranoside (4), 2,6-dimethoxy-4-hydroxyphenol-1-O-β-D-glucoside (5), 4-(3'-hydroxypropyl)-2,6-dimethoxy-phenol- 3'-O-β-D-glcoside (6), thymidine (7), syringin (8), 4-allyl-2,6-dimethoxyphenyl glucoside (9), (+)-syringaresinol (10), syringaresinol- 4'-O-β-D-monoglucoside (11), naringenin (12), p-hydroxybenzoic acid (13), and 4,4'-dihydroxy-3,5-dimethoxybibenzyl (14). Conclusion The compouds 1—6 are isolated from the species of Dendrobium Sw. for the first time, and 7—8 are isolated from D. officinale for the first time.

广西科学研究与技术开发计划项目(桂科转12239002-6);药用资源化学与药物分子工程教育部重点实验室(CMEMR2013-A02)