目的 研究青牛胆Tinospora sagittata的块根中含氮类化学成分及其体外抗肿瘤活性。方法 采用硅胶、ODS、Sephadex LH-20柱色谱等手段分离纯化,根据理化性质和波谱数据鉴定化合物结构。分别采用台盼蓝排斥法和MTT法测试单体化合物对HL-60和MCF-7细胞的抗增殖作用。结果 从青牛胆块根的乙醇提取物中分离得到9个化合物,分别鉴定为四氢巴马汀(1)、四氢药根碱(2)、药根碱(3)、巴马汀(4)、neoechinulin A(5)、echinuline(6)、N-反式阿魏酸酪酰胺(7)、尿嘧啶(8)、三乙胺氢碘酸盐(9)。结论 化合物5、6、8、9为首次从青牛胆属植物中分离得到。化合物1、3～5、7对HL-60细胞显示弱的抗增殖作用。

Objective To investigate the chemical constituents from Tinosporae Radix (the roots of Tinospora sagittata) and their antiproliferative effects in cancer cells. Methods The compounds were isolated and purified by means of silica gel, ODS, and Sephadex LH-20 column chromatography. The structures were identified on the basis of physicochemical properties and spectroscopic analysis. The antiproliferative effects in HL-60 and MCF-7 cells were tested by Trypan blue and MTT methods, respectively. Results Nine compounds were isolated and their structures were identified as tetrahydropalmatine (1), tetrahydrojatrorrhizine (2), jatrorrhizine (3), palmatine (4), neoechinulin A (5), echinuline (6), N-trans-feruloyltyramine (7), uracil (8), and triethylamine hydroiodide (9). Conclusion Among them, compounds 5, 6, 8 and 9 are isolated from the plants of Tinospora Miers for the first time. Compounds 1, 3—5 and 7 show the moderate antiproliferative effects in HL-60 cells.

国家自然科学基金资助项目(81172958)