目的 对青藏大戟Euphorbia altotibetica地上部分的化学成分进行研究。方法 采用多种柱色谱技术进行分离纯化,通过波谱分析鉴定化合物结构。结果 从青藏大戟90%乙醇提取物的醋酸乙酯萃取部分中分离得到6个化合物,分别鉴定为青藏大戟素E(1)、脯氨酸(2)、corchoionoside C(3)、icariside B₂(4)、1-O-(9Z,12Z,15Z-十八烷三烯酰基)-2-O-十六烷酰基-3-O-α-(6-磺基吡喃奎诺糖基) 甘油酯(5)、(2S)-2,3-O-双十八烷-9Z,12Z,15Z-三烯酰基甘油酯-6'-O-(α-D-吡喃半乳糖基)- β-D-吡喃半乳糖苷(6)。结论 化合物1为新的西松烷型二萜,命名为青藏大戟素E;化合物2、5和6为首次从大戟属植物中分离得到,其余化合物为首次从该植物中分离得到。

Objective To study the chemical constituents from the aerial parts of Euphorbia altotibetica. Methods The compounds were isolated and purified by various chromatographic techniques and their structures were elucidated by spectral analysis. Results Six compounds were isolated and their structures were identified as altotibetin E (1), proline (2), corchoionoside C (3), icariside B₂ (4), 1-O-(9Z,12Z,15Z-octadecatrienoyl)-2-O-hexadecanoyl-3-O-α-(6-sulfoquinovopyranosyl) glycerol (5), and (2S)-2,3-O-dioctadeca-9Z, 12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (6). Conclusion Compound 1 is a new cembranoid diterpene named altotibetin E, compounds 2, 5, and 6 are isolated from the species of Euphorbia L. for the first time and the other compounds are firstly obtained from this plant.

科技基础性工作专项重点项目(2012FY110300);国家重点基础研究发展计划(2010CB951704);国家自然科学基金资助项目(31300293);云南省应用基础研究计划面上项目(2013FB067)