目的 建立同时测定疏风解毒胶囊中戟叶马鞭草苷、马鞭草苷、虎杖苷、连翘酯苷A、毛蕊花糖苷、甘草酸单铵盐和大黄素的RP-HPLC方法.方法 采用RP-HPLC法测定,色谱柱为Unitary C₁₈柱(200 mm×4.6 mm,5 μm);流动相为乙腈-0.1%甲酸水溶液,梯度洗脱;体积流量1.0 mL/min;柱温30 ℃;检测波长250 nm;进样量10 μL.结果 戟叶马鞭草苷、马鞭草苷、虎杖苷、连翘酯苷A、毛蕊花糖苷、甘草酸单铵盐和大黄素分别在0.096～1.920、0.089～1.784、0.119～2.348、0.059～1.176、0.021～4.224、0.206～4.120、0.053～1.064 μg与色谱峰面积呈良好的线性关系;平均加样回收率分别为98.43%(RSD 2.13%)、98.30%(RSD 2.68%)、99.31%(RSD 2.76%)、101.64%(RSD 2.37%)、97.47%(RSD 2.05%)、100.89%(RSD 1.98%)、99.05%(RSD 2.21%).结论 该方法简单、快速、准确,为疏风解毒胶囊的质量控制提供一种可靠的参考方法.

Objective To establish a reverse phase high performance liquid chromatographic (RP-HPL) method for the simultaneous determination of the contents of hastatoside, verbenalin, polydatin, ofrsytlloside A, verbascoside, ammonium glycyrrhizinate, and emodin in Shufeng Jiedu Capsule. Methods RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Unitary C₁₈ column (200 mm × 4.6 mm, 5 μm), acetonitrile-0.1% formic acid as the mobile phrase with gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 250 nm, the column temperature was 30 ℃, and the injection volume was 10 μL. Results Linearity relationships of hastatoside, verbenalin, polydatin, ofrsytlloside A, verbascoside, ammonium glycyrrhizinate, and emodin were 0.096—1.920, 0.089—1.784, 0.119—2.348, 0.059—1.176, 0.021—4.224, 0.206—4.120, and 0.053—1.064 μg, respectively, as well as the average recoveries of 98.43% (RSD 2.13%), 98.30% (RSD 2.68%), 99.31% (RSD 2.76%), 101.64% (RSD 2.37%), 97.47% (RSD 2.05%), 100.89% (RSD 1.98%), and 99.05% (RSD 2.21%). Conclusion The method is simple, fast, accurate, and can be used to control the quality of Shufeng Jiedu Capsule.