目的 优化细柱五加Acanthopanax gracilistylus(AGS)叶(AGSL)多糖(AGSL-P)闪式提取工艺,然后将其进行分离纯化,并对其吸湿和保湿性能进行研究.方法 运用球面对称设计试验,优化AGSL-P闪式提取工艺;采用醇沉、Sevage法除蛋白、脱色、透析、琼脂糖凝胶色谱、葡聚糖凝胶分离等方法对AGSL-P进行分离纯化,通过化学实验及红外光谱、核磁共振等方法对其结构进行初步鉴定;对AGSL-P、AGSL-P-1和AGSL-P-2的吸湿和保湿性能进行了初步研究,并与常用保湿剂(聚乙二醇400等)在相对湿度43%和75%条件下进行对比.结果 AGSL-P闪式提取最佳工艺为固液比为1:14.5,提取温度为71 ℃,提取时间为257 s,在此条件下,AGSL-P提取率为1.62%;AGSL-P精制产物AGSL-P-1-1为均一多糖,经过红外光谱和核磁共振检测结果推测,AGSL-P-1-1的构型为α构型,可能含有葡萄糖、鼠李糖和半乳糖.吸湿和保湿性能研究结果表明,AGSL-P-1和AGSL-P-2的吸湿能力优于常用保湿剂聚乙二醇400,而AGSL-P-2保湿能力与聚乙二醇400相当.结论 建立了稳定、简便的AGSL-P闪式提取工艺方法;AGSL-P-1-1均一多糖为首次从AGSL中分离得到;AGSL-P-2可能是一种优良的天然保湿剂

Objective Polysaccharides from the leaves of Acanthopanax gracilistylus (AGSL) were extracted with smashing tissue extraction (STE), isolated and purified, and the properties of their moisture retention and moisture absorption were studied. Methods Extraction process for AGSL polysaccharides (AGSL-P) was optimized by using the spherical symmetrical design test, and then isolated and purified through alcohol precipitation, Sevage method, bleaching, dialysis, DEAE-Sepharose Fast Flow column, and Sephadex G-75 column, and the structure was identified by chemical experiment, IR, and NMR. The properties of the moisture retention and moisture absorption of AGSL-P, AGSL-P-1, and AGSL-P-2 were studied and compared with common humectants (polyethylene glycol 400 etc.) in relative humidity 43% and 75%. Results The optimal STE conditions for AGSL-P were as follows: material-liquid ratio was 1:14.5, the extraction temperature was 71 ℃, and the extraction time was 257 s. With the best extraction conditions, the yield of AGSL-P was 1.62%. AGSL-P-1-1 was homogeneous polysaccharide with α-configuration, and it may contain glucose, rhamnose, and galactose. The results showed the absorbent capacity of AGSL-P-1 and AGSL-P-2 was superior to the common humectants, polyethylene glycol 400, and moisturizing ability of AGSL-P-2 was amount to polyethylene glycol 400. Conclusion A steady and convenient extraction technology for AGSL-P has been established using STE method, AGSL-P-1-1 homogeneous polysaccharide is isolated from the leaves of AGSL has been established using STE method, AGSL-P-1-1 is isolated from the leaves of AGSL for the first time, and AGSL-P-2 is an excellent moisturizer.

湖南省自然科学基金重点项目(11JJ2042);湖南中医药大学药物分析学“十二五”校级重点学科建设项目