目的 筛选北细辛Asarum heterotropoides var. mandshuricum愈伤组织诱导适宜条件,确定愈伤组织类型.方法 以北细辛叶柄和叶片为材料,研究外植体状态、培养基类型及植物生长调节剂种类和浓度配比对愈伤组织诱导发生的影响,同时利用石蜡切片法探讨不同时期愈伤组织的形态结构特点.结果 以幼期叶柄和叶片为外植体,高效的愈伤组织诱导培养基为1/2 MS＋0.6 mg/L 6-BA＋0.15 mg/L NAA;但幼期叶片愈伤组织发生所需时间较长.切片观察发现诱导形成的愈伤组织结构紧密,细胞小且排列无规则,为胚性愈伤组织.结论 幼期叶柄是北细辛愈伤组织诱导的最佳外植体材料,获得的高效愈伤组织诱导条件将为后续再生体系的成功建立奠定基础.

Objective To optimize the proper condition for callus induction of Asarum heterotropoides var. mandshuricum. Methods The effects of different explants, medium types, and plant growth regulators on the callus induction of A. heterotropoides var. mandshuricum were studied by using the petioles and leaves as explants. Also we aimed to discover the morphology characteristics of callus in different periods through paraffin method. Results The efficient callus induction medium was 1/2 MS + 0.6 mg/L 6-BA + 0.15 mg/L NAA while using petioles and leaves during young period as explants. Callus induction from young leaves lasted so long time. Furthermore it was found that the induced calli were embryogenic for they were composed of compact, small and irregular arrangement cells. Subsequently these calli could be differentiated to develop the regeneration plantlet. Conclusion Young petiole is the optimal explant for callus induction of A. heterotropoides var. mandshuricum, and the efficient callus induction condition we optimized would provide a basis for establishing the subsequent regeneration system.

黑龙江省教育厅科学技术研究项目计划(12531030)