目的 建立从金银花Lonicera japonica中分离制备高质量分数异绿原酸A、B和C的方法.方法 采用D-101大孔树脂、中低压制备色谱分离制备金银花中异绿原酸A、B和C单体,根据理化性质和波谱数据鉴定其结构.结果 分离制备的异绿原酸A、B和C质量分数分别为98.7%、99.2%和97.6%.结论 该方法经济、简单、快速,可用于制备高质量分数的异绿原酸A、B和C.

Objective To establish a method for separation of isochlorogenic acids A, B, and C from Lonicerae Flos. Methods Isochlorogenic acids A, B, and C in Lonicerae Flos were isolated and purified by macroporous resin and medium-low-pressure preparative chromatography. Their structures were identified on the basis of the spectral data and physicochemical property. Results The contents of prepared isochlorogenic acids A, B and C were 98.7%, 99.2%, and 97.6%, respectively. Conclusion This method is economic, simple, rapid, and effective for the preparation of isochlorogenic acids A, B, and C with high purity.

科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)