目的 观察参附汤有效组分及其配伍对大鼠心室肌细胞L型钙通道电流的作用.方法 采用Langendorff心脏灌流,急性酶解法获得单个心室肌细胞,全细胞膜片钳技术记录并分析参附汤人参有效组分人参皂苷Re、Rg₁、Rb₁和附子有效组分乌头原碱及其配伍对心肌细胞L型钙通道电流的影响.结果 人参皂苷Re和Rg₁能够可逆性地阻断L型钙通道电流,给予不同浓度人参皂苷Re(3、10、30、100 μmol/L)和Rg₁(10、100 μmol/L)后L型钙通道电流幅度呈现随药物浓度增加逐渐减小的趋势;人参皂苷Rb₁(10、100 μmol/L)对L型钙通道电流幅度变化无明显影响;乌头原碱能够不可逆地增强L型钙通道电流,给予不同浓度乌头原碱(10、100 μmol/L)后L型钙通道电流幅度随药物浓度增加逐渐增大.联合使用人参皂苷Re(20 μmol/L)和Rg₁(80 μmol/L)对L型钙通道的抑制作用强于单独使用人参皂苷Re和Rg₁;而人参皂苷Rg₁(80 μmol/L)、Re(20 μmol/L)和乌头原碱(100 μmol/L)联合应用对人参皂苷Rg₁和Re配伍对L型钙通道电流的抑制作用无明显影响.结论 参附汤有效组分人参皂苷Rg₁、Re抑制L型钙通道电流,而乌头原碱对L型钙通道电流有不可逆的增强作用,人参皂苷Rg₁、Re配伍对L型钙通道电流的抑制作用增强,乌头原碱配伍后对人参皂苷Rg₁、Re的L型钙通道电流抑制作用无明显影响.

Objective To investigate the regulation of in vitro myocardial cell L type calcium channel of rats with active component of Shenfu Decoction and its combination. Methods With Langendorff cardiac infusion, the single ventricular myocardial cell with acute enzymylosis approach was acquired and the voltage current of L type calcium channel was recorded with whole cell patch technique. Results The active ginsenosides Re (3, 10, 30, and 100 μmol/L), and Rg₁ (10 and 100 μmol/L) can reversibly reduce L type calcium current in a dose dependant manner, Rb₁ (10 and 100 μmol/L) has no effect on L type calcium current; the active component aconine (10 and 100 μmol/L) of aconite could irreversibly enhance L type calcium current in a dose dependant manner; The combination of ginsenosides Re (20 μmol/L) and Rg₁ (80 μmol/L) also reduced the L type calcium current, which is more significant than either ginsenosides Rg₁ and Re; The inhibitory rate of the combination of ginsenosides Rg₁, Re and aconine (100 μmol/L) has no difference from the combination of ginsenosides Rg₁ and Re. Conclusion The active component ginsenosides Rg₁ and Re of Shenfu Decoction can reduce the L type calcium current, while aconine can irreversibly enhance the L type calcium current; The combination of ginsenosides Rg₁ and Re can obviously increase the reduction of L type calcium current, while the combination of ginsenosides Rg₁, Re and anonine can not change the reduction of L type calcium current.

教育部中央高校基金项目(JYBZZ－JS005);教育部留学回国人员科研启动基金