目的 研究福寿草Adonis amurensis带根全草的化学成分.方法 运用硅胶、凝胶、小孔树脂及PR-HPLC等多种色谱技术对福寿草带根全草的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构.结果 从福寿草带根全草95%乙醇提取物的醋酸乙酯和正丁醇萃取部位分离鉴定了15个化合物,其中强心苷类化合物7个,分别为毒毛旋花子苷元(1)、铃兰毒苷(2)、铃兰毒原苷(3)、索马林(4)、洋地黄毒苷元(5)、罗布麻苷(6)、叶含杠柳鼠李糖苷(7);黄酮类化合物5个,分别为木犀草素(8)、芹菜素(9)、芹菜素-7-O-β-D-葡萄糖醛酸(10)、荭草苷(11)、异槲皮苷(12);木脂素类化合物3个,分别为松脂素(13)、松脂素-8-O-β-D-葡萄糖苷(14)、9'-脱羧迷迭香酸-4'-O-(1→4)-半乳糖鼠李糖苷(15).结论 化合物10、13～15是首次从该属植物中分离得到,化合物3、10、13～15为首次从该植物中分离得到.

Objective To study the chemical constituents from herb with the roots of Adonis amurensis. Methods The compounds were isolated and purified by silica gel column, Sephadex LH-20, MCI, and reverse phase HPLC chromatographies, and the structrures of the isolated compounds were elucidated by spectral analysis and physicochemical properties. Results Fifteen compounds were isolated from EtOAc and n-butanol fraction of the 95% ethanol etract in the herb with the roots of A. amurensis, including seven cardenolides, i.e. strophanthidin (1), convallatoxin (2), convalloside (3), somalin (4), digitoxigenin (5), cymarin (6), and periplorhamnoside (7); five flavonoids, i.e. luteolin (8), apigenin (9), apigenin-7-O-β-D-glucuronide (10), orientin (11), and isoquercitrin (12); and three lignans, i.e. pinoresinol (13), pinoresinol-8-O-β-D-glucopyranoside (14), and 9'-decarboxy rosmarinic acid-4'-O-(1→4)-galactosyl rhamnoside (15). Conclusion Compounds 10 and 13-15 are firstly isolated from the plants of Adonis L. and compounds 3, 10, and 13-15 are isolated from A. amurensis for the first time.

广东省自然科学基金团队项目(8351063201000003)