目的 建立HPLC法同时测定桂枝芍药知母汤中没食子酸、芒果苷、芍药苷、苯甲酸和肉桂酸的量。方法 采用Zorbax Extend-C₁₈(150 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-水梯度洗脱,体积流量为1.0 mL/min,检测波长为230 nm,柱温为25 ℃。结果 没食子酸、芒果苷、芍药苷、苯甲酸和肉桂酸进样量分别在0.052～0.52(r=0.999 8)、0.078～0.78(r=0.999 8)、0.336～3.36(r=0.999 9)、0.029 6～0.296(r=0.999 8)、0.020 6～0.206(r=0.999 9)μg线性关系良好;平均回收率(RSD)分别为99.73%(1.63%)、99.33%(1.02%)、100.2%(1.79%)、98.96%(1.02%)和99.64%(1.62%)。结论 本方法简便、可靠、重复性好,为桂枝芍药知母汤物质基础研究提供了依据。

Objective To establish an HPLC method for the determination of galic acid, mangiferin, paeoniflorin, benzoic acid, and cinnamic acid in Guizhi Shaoyao Zhimu Decotion (GSZD). Methods The separation was performed on a Zorbax Extend-C₁₈ (150 mm × 4.6 mm, 5 μm) column with the gradient elution of acetonitrile-water; The volumn flow was 1.0 mL/min; The detection wavelength was set at 230 nm. The colunm temperature was maintained at 25 ℃. Results Galic acid, mangiferin, paeoniflorin, benzoic acid, and cinnamic acid had good linearity in the ranges of 0.052-0.52 (r = 0.999 8), 0.078-0.78 (r = 0.999 8), 0.336-3.36 (r = 0.999 9), 0.029 6-0.296 (r = 0.999 8), and 0.020 6-0.206 (r = 0.999 9) μg, respectively. The average recoveries of galic acid, mangiferin, paeoniflorin, benzoic acid, and cinnamic acid were 99.73% (1.63%), 99.33% (1.02%), 100.2% (1.79%), 98.9% (1.02%), and 99.64% (1.62%), respectively. Conclusion This method is simple, reliable, and accurate, and can provide the reference for study on GSZD material basis.

中央级科研院所自主选题(YZ-1323)