目的 研究益母草注射液的化学成分。方法 采用大孔吸附树脂、硅胶柱色谱和凝胶柱色谱等方法进行分离纯化,根据理化性质及波谱数据鉴定化合物结构。结果 从益母草注射液中分离鉴定了15个化合物,包括氨基酸及其衍生物4个:N-异丁基缬氨酸(1)、缬氨酸(2)、丙氨酸(3)、L-焦谷氨酸甲酯(4);生物碱类9个:水苏碱(5)、胆碱(6)、葫芦巴碱(7)、尿嘧啶(8)、5-甲基尿嘧啶(9)、2', 3'-O-异亚丙基尿苷(10)、3-羟基吡啶(11)、5-羟基-2-羟甲基吡啶(12)、2-甲基- 3-羟基吡啶(13);呋喃羧酸2个:2-呋喃甲酸(14)、5-羟甲基-2-呋喃甲酸(15)。结论 化合物2～4、6～7、9～15为首次从益母草属植物中分离得到,其中化合物1为新天然产物,并首次报道了其NMR数据。

Objective To study the chemical constituents from Leonurus japonicus Injection. Methods The constituents were purified by macroporus resin, silica gel, Sephadex LH-20, and recrystallization. The structures were identified by spectroscopic methods and spectroscopic analysis. Results Fifteen compounds were isolated from L. japonicus Injection, including four amino acids and their derivatives such as N-isobutyl valine (1), valine (2), alanine (3), L-pyroglutamic acid methyl ester (4), nine alkaloids: stachydrine (5), choline (6), trigonelline (7), uracil (8), 5-methyluracil (9), 2', 3'-O-isopropylidene uridine (10), 3-hydroxypyridine (11), 5-hydroxyl-2-hydroxymethylpyridine (12), and 2-methyl-3-hydroxypyridine (13); and two furoic acids such as furan-2- carboxylic acid (14) and 5-hydroxymethyl-2-furancarboxyic acid (15). Conclusion In addition to compounds 5 and 8, the others are isolated from the plants of Leonurus Linn. for the first time. Among them, compound 1 is a natural product. Its NMR data, undoubtedly assigned by 2D-NMR, have been reported for the first time.

国家自然科学基金资助项目(81303209);中国博士后科学基金项目(2014M562291);四川省应用基础研究项目(2012JYZ005);四川省科技支撑计划(2011SZ0056);成都中医药大学校基金(ZRQN1469)