目的 建立HPLC法同时测定丹芎通脉颗粒中丹酚酸B、阿魏酸、羟基红花黄色素A、没食子酸和咖啡酸的方法。方法 采用Thermo ODS-2 Hypersil（250 mm×4.6 mm，5 μm）色谱柱，以0.1%乙酸水溶液-甲醇为流动相，梯度洗脱，体积流量1.0 mL/min，检测波长为286 nm，柱温30 ℃。结果 丹芎通脉颗粒中丹酚酸B、阿魏酸、羟基红花黄色素A、没食子酸和咖啡酸分别在3.18～50.88 μg/mL（r=0.999 0）、2.00～32.00 μg/mL（r=0.999 5）、6.00～96.00（r=0.999 6）、0.60～9.60 μg/mL（r=0.999 4）和1.00～16.00 μg/mL（r=0.999 5）与峰面积呈良好线性关系；平均回收率分别98.12%、97.23%、98.51%、99.11%和97.01%（n=6）。结论 该方法准确可靠，简单、快速，重复性好，可用于丹芎通脉颗粒中丹酚酸B、羟基红花黄色素A、阿魏酸、没食子酸和咖啡酸成分的质量控制。

Objective To establish an HPLC method for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule. Methods The determination was carried out by HPLC, using Thermo ODS-2 Hypersil (250 mm × 4.6 mm, 5 μm) chromatographic column. The mobile phase was 0.1% acetic acid solution-methyl alcohol, gradient elution, and UV detection wavelength was set at 286 nm. The column temperature was set at 30 ℃. The injection volume was 10 μL. Results Under the above chromatographic conditions, there was a good linearity between the absorption peak area and the concentration in the ranges of 3.18—50.88 μg/mL (n= 0.999 0) for salvianolic acid B, 2.00—32.00 μg/mL (n= 0.999 5) for fumalic acid, 6.00—96.00 (n= 0.999 6) for hydroxysafflor yellow A, 0.60—9.60 μg/mL (n= 0.999 4) for gallic acid, and 1.00—16.00 μg/mL (n= 0.999 5) for caffeic acid, respectively. Conclusion This method is simple, fast, accurate, reliable, and reproducible, and it is proper for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule at the same time.

国家自然科学基金资助项目（81274005）;河北省卫生厅项目（20090588，20110173）;河北省教育厅项目（Z2011304）