目的 制备鲜马齿苋多糖、生物碱和多酚类组分，并研究各组分的降血糖活性。方法 选择合适的树脂和优化的分离纯化工艺，富集鲜马齿苋中多糖、生物碱、多酚类组分；并用苯酚-硫酸法测定多糖组分的量，HPLC法测定生物碱和多酚类组分的量。采用链脲佐菌素（STZ）诱导的糖尿病小鼠模型，分析多糖、生物碱、多酚类组分对糖尿病小鼠血糖、血清胰岛素和肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）的影响。结果 鲜马齿苋多糖、生物碱及多酚类组分均能降低糖尿病小鼠血糖、促进胰岛素分泌，多糖类组分和多酚类组分能明显提高肝组织中SOD活性、降低MDA水平，具有明显的抗氧化作用，且多糖、生物碱及多酚3类组分整体给药使糖尿病小鼠血糖下降率最显著。结论 分离、纯化所得的鲜马齿苋多糖、生物碱和多酚类组分均具有明显降血糖活性，且鲜马齿苋多糖、生物碱和多酚3类组分整体给药降血糖效果更佳。

Objective To prepare polysaccharides, alkaloids, and polyphenols from fresh Portulaca oleracea and study their hypoglycemic effects. Methods The suitable macroporous resin and adequate separation and purification technology were selected to prepare polysaccharides, alkaloids, and polyphenols from fresh P. oleracea; Then their hypoglycemic effects were studied by analyzing fasting blood serum, insulin, and MDA and SOD from STZ induced diabetic mice. Results All these three compositions can low fasting blood serum of diabetic mice. What's more, polysaccharides and polyphenols can obviously improve the content of SOD and degrade the content of MDA, while alkaloids can promote pancreatic β echylosis. Conclusion Polysaccharides, alkaloids, and polyphenols from fresh P. oleracea possess anti-diabetic effects, and the integral administration of the three compositions has better hypoglycemic effect.

江苏省中医药领军人才项目（2006）