目的 采用二次通用旋转组合设计优化黄芪中毛蕊异黄酮苷和芒柄花素的酶解提取工艺。方法 在确定酶比例的基础上，选定复合酶用量、酶解时间、加水量和提取时间为考察因素，以毛蕊异黄酮苷、芒柄花素提取量为指标，采用二次通用旋转组合设计优化黄芪酶解提取工艺。结果 优化所得黄芪酶解提取的最佳工艺条件：复合酶用量340 mg，酶解时间110 min，加水量19倍，提取时间150 min，在此条件下，毛蕊异黄酮苷、芒柄花素提取量分别为0.25 mg/g、67.95 mg/g。结论 优化所得黄芪酶解提取工艺稳定可行。

Objective The technological conditions for enzymatic extraction of calycosin glucoside and formononetin in Astragali Radix were optimized by using quadratic general rotary unitized design. Methods On the base of single factors experiments, main factors that affecting the extraction efficiency, such as enzyme dosage, enzymatic hydrolysis time, adding water volume, and extraction time were selected. With the content of calycosin glucoside and formononetin as evaluation index, the process of enzymatic extraction of Astragali Radix was optimized by using quadratic general rotary unitized design. Results The optimum enzymatic extraction process was as follows: enzyme dosage 340 mg, enzymatic hydrolysis time 110 min, adding water volume 19 times, and extraction time 150 min. Under these optimum conditions, the content of calycosin glucoside was 0.25 mg/g, and formononetin was 67.95 mg/g. Conclusion The optimum enzymatic extraction process of Astragali Radix is stable and feasible.

国家自然科学基金资助项目（81060345）