[关键词]
[摘要]
目的 对25种不同品牌、产地和年份的国产干红葡萄酒中7种活性成分(没食子酸、原儿茶酸、儿茶素、表儿茶素、原花青素B2、白藜芦醇和鞣花酸)进行HPLC法同步检测,为其质量评价提供科学依据。方法 采用HPLC法测定中国干红葡萄酒中没食子酸、原儿茶酸、儿茶素、表儿茶素、原花青素B2、白藜芦醇和鞣花酸的量。色谱条件为Kromasil C18(150 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱:0~21 min,19%乙腈;21~24 min,19%~25%乙腈;24~32 min,25%~30%乙腈;32~39 min,30%~40%乙腈;39~44 min,40%~50%乙腈;44~55 min,50%~60%乙腈;55~60 min,60%~80%乙腈;60~65 min,80%~100%乙腈;65~70 min,100%乙腈;体积流量为1 mL/min;柱温25 ℃;检测波长为280 nm;每次进样10 μL。结果 实验所测中国干红葡萄酒中都含有没食子酸、原儿茶酸、儿茶素、表儿茶素、原花青素B2、白藜芦醇和鞣花酸7种活性成分,定量范围依次为31.6~111.9、3.7~11.9、30.7~71.9、30.3~135.7、29.8~82.5、0.9~12.3、1.9~23.2 mg/L。结论 没食子酸、原儿茶酸、儿茶素、表儿茶素、原花青素B2、白藜芦醇和鞣花酸7种活性成分可作为国产干红葡萄酒质量评价的指标,它们的存在是干红葡萄酒品质的保障和保健作用的物质基础。
[Key word]
[Abstract]
Objective Using HPLC to simultaneously detect the phenolic compounds in active ingredients, such as gallic acid, protocatechuic acid, catechin, epicatechin, proanthocyanidin B2, resveratrol, and ellagic acid, in 25 kinds of Chinese dry red wines with different brands, from different places, and in different years, and to provide the foundation for the quality assessment. Methods HPLC was applied to determinating the content of each phenonic ingredient. The HPLC conditions were as follows: chromatographic column used as Kromasil C18 (150 mm × 4.6 mm, 5 μm), mobile phase was methol (A)-H2O-0.1% phosphoric acid (B). The gradient program was 0-21 min, 19% A; 24 min, 25% A; 32 min, 30% A; 39 min, 40% A; 44 min, 50% A; 55 min, 60% A; 60 min, 80% A; 65-70 min, 100% A. Mobile flow rate with 1 mL/min and the detective wavelength was 280 nm. Results The contents of gallic acid, protocatechuic acid, catechin, epicatechin, proanthocyanidin B2, resveratrol, and ellagic acid in these wines were in the ranged of 31.6-111.9, 3.7-11.9, 30.7-71.9, 30.3-135.7, 29.8-82.5, 0.9-12.3, and 1.9-23.2 mg/L, respectively. Conclusion The seven phenolic compounds could be selected as the index ingredients to evaluate the quality of dry red wine from the different sources and the existence of them are guarantee of quality of dry red wine and the material basis of healthcare functions.
[中图分类号]
[基金项目]
沈阳市科技计划项目(F11-153-9-00);辽宁省博士启动基金资助项目(20111135)