目的 优化痛安注射液组方药材白屈菜提取物精制纯化工艺，提高白屈菜提取物中白屈菜碱的量。方法 分别考察平板直联式离心、高速管式离心、碟片离心不同离心技术方法，对白屈菜酸沉液分离的效果；分别考察不同pH值酸水液对白屈菜萃取干膏溶解效果的影响；分别考察减压滤过、高速管式离心、平板直联式离心不同分离方式对白屈菜酸水溶解液分离的效果；以白屈菜碱转移率、干膏得率为评价指标，确定可行的白屈菜碱精制纯化工艺。结果 采用碟片离心技术对白屈菜酸沉液分离效果最好；酸水液pH值在3～4时酸溶解效果及白屈菜碱转移率最佳；高速管式离心对白屈菜酸水溶解液分离效果最好。结论 该工艺制备白屈菜提取物，操作简单，稳定可行。

Objective To optimize the purification process of celandine in Celandine Alkali from Tong'an Injection, and to improve the content of celandine in Celandine Alkali extract. Methods In order to select the best way to separate celandine acid liquid, a study was carried out in three different ways of separation such as plate straight association-like centrifuge, high speed tubular centrifuge, and disc centrifuge; We investigated the dissolving effect caused by different pH values of hydrochloric acid to celandine dry extract; Three separation methods on celandine acidic lysate were investigated to choose the best, such as vacuum filtration, high-speed centrifuge tube, and flat direct-coupled centrifugal. Celandine Alkali transfer rate, and dry paste rate were as used evaluation indexes, so as to determine the feasible celandine refining purification process. Results It was the best way to separate celandine acid liquid by using disccentrifuge; Hydrochloric acid could have the best dissolving effect on celandine dry extract when the pH values were 3—4, and the transfer rate of chelidonine was also the highest; High-speed tubular centrifuge was the best separation method for the celandine acidic dissolution liquid. Conclusion The optimized process is simple, stable and viable, and it could be used for the preparation of qualified celandine intermediates.

科技部重大新药创制：现代中药创新集群与数字制药技术平台（2013ZX09402203）