目的 探讨高良姜素分子印迹聚合物（GMIP）的特异性分子识别能力。方法 以高良姜素为模板分子，分别采用四氢呋喃、乙腈、丙酮为致孔剂，以丙烯酰胺（AM）为功能单体，以乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，采用热引发聚合的方法合成GMIP；采用扫描电子显微镜（SEM）、傅里叶红外光谱（FTIR）扫描对其进行性能表征；运用平衡结合试验研究聚合物的吸附特性和选择识别能力。结果 Scathard模型分析表明，该GMIP在识别高良姜素分子的过程中存在2类不同的结合位点；高亲和力结合位点的离解常数（K_d1）=0.961 mmol/L，最大表观结合常数（Q_max1）=19.79 μmol/g；低亲和力结合位点的离解常数（K_d2）=0.101 mmol/L，最大表观结合常数（Q_max2）=51.09 μmol/g。结论 GMIP对高良姜素分子具有特异的吸附和识别能力，为天然产物中高效分离纯化活性成分高良姜素提供了一种新型材料。

Objective To investigate the specific molecular recognition ability for galangin molecular imprinting polymer (GMIP). Methods GMIP was prepared through the method of thermal polymerization with galangin as template molecular, acrylamide (AM) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as cross-linker, and tetrahydrofuran, acetonitrile, and acetone as pore forming agents, respectively. The GMIP was characterized by infrared spectroscopy and scanning electron microscopy (SEM). In addition, the GMIP was investigated in equilibrium binding experiment to evaluate its adsorption property and selective recognition. Results The Scathard model analysis showed that two kinds of binding sites existed in the GMIP. The dissociation constant (K_d) and apparent maximum binding constant (Q_max) were K_d1 = 0.961 mmol/L, Q_max1 = 19.79 μmol/g for high affinity binding sites and K_d2 = 0.101 mmol/L, Q_max2 = 51.09 μmol/g for low affinity binding sites, respectively. Conclusion GMIP has the specific adsorption and recognition capabilities to the galangin molecules. It can be a novel material for the separation and purification of the active ingredients from natural products.

山西医科大学科技创新基金资助项目（C01201005）；山西医科大学青年基金资助项目（02201309）