目的 采用组织培养快繁技术培养五指毛桃种苗，为人工种植提供种源。方法 采用五指毛桃叶片作外植体，以MS和1/2 MS为基本培养基，采用正交设计研究植物生长调节剂多因素组合(6-BA、NAA、2, 4-D、IAA、KT和IBA)对五指毛桃初代诱导、不定芽分化和诱导生根的影响。结果 不定芽最佳诱导培养基为MS＋1.0 mg/L 6-BA＋0.3 mg/L NAA，外植体经20 d诱导培养，可分化形成不定芽72个；MS＋1.0 mg/L 6-BA＋0.3 mg/L IAA＋0.3 mg/L KT最利于不定芽继代增殖，增殖倍数6.67；1/2 MS＋1.0 mg/L IBA＋0.3 mg/L NAA最适于诱导生根获得再生植株，生根率100%；宜移栽于泥炭-珍珠岩(1：1)的基质上，成活率为93%。结论 此途径繁殖速度快、再生率高，能为栽培五指毛桃提供大量种苗。

Objective To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture. Methods The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator (6-BA, NAA, 2, 4-D, IAA, KT, and IBA) on the seedling subculture and root culture was studied by orthogonal design. Results The best medium for the adventitious bud induction was MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS + 1.0 mg/L 6-BA + 0.3 mg/L IAA + 0.3 mg/L KT; The best rooting medium was 1/2 MS + 1.0 mg/L IBA + 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite (1:1) matrix, the 30 d survival rate was 93%. Conclusion The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting.

广西壮族自治区卫生厅中医药科技专项（GZKZ10－081）