目的 研究水忍冬Lonicera dasystyla藤茎的活性物质基础。方法 采用硅胶柱色谱、凝胶Sephadex LH-20柱色谱及ODS反相柱色谱等进行分离纯化，通过质谱、核磁共振波谱技术等分析手段进行结构鉴定。结果 首次从水忍冬藤茎醇提浸膏的醋酸乙酯萃取部位分离得到9个化合物，分别鉴定为二氢芝麻素-9-O-β-D-吡喃葡萄糖苷(1)、异阿魏酸(2)、对甲氧基香豆酸(3)、山柰酚-7-O-(2”-E-对香豆酰基)-α-L-吡喃阿拉伯糖苷(4)、灰毡毛忍冬皂苷乙(5)、马钱子苷(6)、山柰酚(7)、合金欢素(8)、槲皮素(9)。结论 化合物1为新化合物，命名为二氢芝麻素-9-O-β-D-吡喃葡萄糖苷，根据文献报道，化合物5、6为该属植物的特征成分；化合物2～4及8为首次从该属植物中分离得到。这些化学成分的分离鉴定为研究水忍冬藤茎的活性物质奠定了基础，对该植物的化学分类学有一定的指导意义。

Objective To search for the bioactive compounds from the vine of Lonicera dasystyla. Methods The compounds were isolated and purified by column chromatography on silica gel, sephadex LH-20 gel, and ODS, and their structures were elucidated on the basis of spectroscopic analysis, such as MS, NMR, etc. Results Nine compounds were isolated from the vine of L. dasystyla. Their structures were identified as dihydrosesamin-9-O-β-D-glucopyranoside (1), isoferulic acid (2), p-methoxycoumaric acid (3), kaempferol-7-O-(2"-E-p-coumaroyl)-α-L-arabinofuranoside (4), macranthoidin B (5), loganin (6), kaempferol (7), acacetin (8), and quercetin (9). Conclusion All of these compounds belong to four types, phenylpropanoids (1—3), flavonoids (4, 7—9), triterpenoid saponin (5), and iridoid (6). Compound 1 is a new one and compounds 5 and 6 as marker compounds exist in most of Lonicera Linn. plants, while known compounds 2—4 and 8 were isolated from Lonicera Linn. plants for the first time. The identification of these chemicals lays the foundation for searching bioactive compounds of this plant, and these compounds have great importance for chemotaxonomy of this plant.

广西壮族自治区科技计划项目（桂科合-1298014-4）；广西自然科学基金重点项目（2011GXNSFD018037）