目的 建立以HPLC法测定六味木香胶囊中木香烃内酯和去氢木香内酯的方法。方法 色谱柱为VP-ODS柱（250 mm×4.6 mm，5 μm），流动相为甲醇-水（72：28），检测波长为225 nm，柱温为25℃，体积流量为1.0 mL/min。结果 木香烃内酯、去氢木香内酯进样量分别在203.12～2 031.20、207.84～2 078.40 ng与峰面积积分值呈良好的线性关系（r=0.999 9）；平均回收率分别为101.1%、101.2%，RSD分别为0.48%、0.73%（n=6）。结论 建立的方法简便、准确、重复性好、专属性强，可用于该制剂的质量控制和评价。

Objective To establish an HPLC method for the determination of costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. Methods The determination was performed on VP-ODS (250 mm × 4.6 mm, 5 μm) with column temperature set at 25℃. The mobile phase consisted of methanol-0.4% phosphoric acid water (72:28) at a flow rate of 1.0 mL/min. The detection wavelength was set at 225 nm. Results The linear range of costunolide and dehydrocostuslactone was 203.12-2 031.20 ng and 207.84-2 078.40 ng (r=0.999 9), with average recovery at 101.1% and 101.2%, RSD=0.48% and 0.73% (n=6). Conclusion The method proved is simple, accurate, reproducible, and specific, and it can be used for the quality control and evaluation of Liuwei Muxiang Capsules.