目的 优选管花肉苁蓉Cistanche tubulosa中苯乙醇苷的提取纯化工艺。方法 以松果菊苷和麦角甾苷质量分数为考察指标，采用L₉（3⁴） 正交试验设计，对提取工艺进行优化；考察7种大孔树脂对2种苯乙醇苷的吸附和解吸附性能，确定最佳纯化工艺条件。结果 最佳提取纯化工艺为管花肉苁蓉药材加12倍量50%乙醇，70℃温浸2次，温浸时间1 h，滤过，合并滤液，回收溶剂，加水调整为含生药0.2 g/mL的上样溶液，上样量为0.8倍柱体积（BV），大孔树脂柱的径高比为1：9，先用3 BV的水除杂，再用4 BV的30%乙醇洗脱，洗脱液减压浓缩至干，即得肉苁蓉苯乙醇苷纯化物，该纯化物中2种苯乙醇苷质量分数>75%。结论 优选得到的提取纯化工艺稳定可行，适合工业化生产。

Objective To optimize the extraction and purification technology of phenylethanoid glycoside from Cistanche tubulosa. Methods Orthogonal design L₉(3⁴) was employed to optimize the extraction conditions by taking the extraction yield of echinacoside and acteoside as indexes. The absorption-desorption characteristics of seven macroporous resins were evaluated, then the best purification conditions were optimized. Results The optimal extraction conditions were as follows: The air-dried stems of C.tubulosa were powdered and extracted twice with 12-fold 50% ethanol for 1 h each time, temperature was 70℃; The supernatant was concentrated to 5-fold weight of the stems of C.tubulosa. The concentrated liquid was subjected to macroporous resin (HPD750) column and then eluted with deionezed water (3 BV) and 30% ethanol (4 BV), respectively. The 30% ethanol fraction was evaporated under vacuum to give the phenylethanoid glycoside-rich C.tubulosa stem extract. The purity of phenylethanoid glycoside was above 75%. Conclusion The optimized extraction and purification process is stable, efficient, and suitable for industrial production.

四川省教育厅资助项目（13ZB0304）；成都中医药大学科技发展基金（ZRMS201246）