目的 采用血液微透析技术进行丹参水煎液清醒动物多次ig给药的药动学研究。方法 以丹参水煎液为工具药物，以丹参素为评价指标，以清醒家兔为实验动物，结合微透析采样技术和液质检测技术，进行日内多次给药的药动学研究，以非房室模型获取药动学参数。结果 建立了基于微透析法的清醒动物汤剂多次给药的药动学研究模式，完整采集了整个过程的药动学信息，方法稳健、可靠、简便。结论 此模式可为类似研究提供借鉴。

Objective To carry out the pharmacokinetic study in awake animal after multiple dosing of Danshen（Salvia miltiorrhiza) Decoction by blood microdialysis method combined with LC-MS. Methods The pharmacokinetic study was carried out with awake rabbits as experimental animal, Danshen Decoction as tool drug, and Danshensu as evaluation index combining with microdialysis sampling and LC-MS detection technology after intraday multiple dosing. The pharmacokinetic parameters were obtained by non-compartmental model method. Results The pharmacokinetic study pattern of multiple dosing was established in awake animal, and the whole process dynamic characteristics were collected. The method was stable, reliable, and simple. Conclusion This study can make the reference to similar study for other drugs.

国家自然科学基金资助项目（30701097）