目的 研究中药提取物对氧化应激诱导的果蝇肠道免疫功能的影响。方法 通过喂食氧化应激物（百草枯和H₂O₂）后统计果蝇生存率的方法，从50种中药中筛选出具有缓解氧化应激损伤作用的中药。通过分析果蝇寿命、肠道干细胞和成肠细胞数量等，探索中药对果蝇肠道氧化应激损伤的影响。结果 瓜蒌、红花、川芎和菊花具有缓解氧化应激损伤的作用，主要表现为提高百草枯和H₂O₂诱导后果蝇的生存率、延长果蝇寿命、缓解由老化及百草枯诱导的肠道干细胞和成肠细胞数量过多的现象。结论 瓜蒌、红花、川芎和菊花提取物对百草枯和H₂O₂诱导的氧化应激损伤具有很好的缓解和保护作用。

Objective To study the effect of the extract from Chinese materia medica (CMM) on oxidative stress-induced gut immunity of Drosophila melanogaster. Methods D. melanogaster was treated by Paraquat and H₂O₂ with or without CMM, and the effects of 50 kinds of CMM on the survival rate of D. melanogaster in response to oxidative stress were recorded. And the antioxidant effects of CMM on lifespan and the number of ISC and enteroblast (EB) in intestinal epithelium cells were analyzed. Results The extracts from Trichosanthes kirilowii (Trichosathis Fructus), Carthamus tinctorius (Carthami Flos), Ligusticum chuanxiong (Chuanxiong Rhizoma), and Chrysanthemum monfolium (Chrysanthemi Flos) could significantly improve the survival rate of Paraquat and H₂O₂-induced D. melanogaster, increase the lifespan of D. melanogaster, and regulate Paraquat-induced and age-related changes in the number of ISC and EB. Conclusion The extracts from T. kirilowii, C. tinctorius, L. chuanxiong, and C. monfolium could significantly improve the oxidative stress-induced gut immunity of D. melanogaster.

国家自然科学基金资助项目（31270923）；中央高校基本科研业务费专项资金（DL13EA08-01）