目的 建立藏药坐珠达西中8种成分的HPLC定量测定方法，提高藏药坐珠达西HPLC检测标准。方法 采用HPLC法，Kromasil C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相为甲醇-0.05%磷酸水溶液，梯度洗脱，体积流量1 mL/min，检测波长：没食子酸、苯甲酸、山柰酚、木香烃内酯和去氢木香烃内酯检测波长为225 nm，绿原酸检测波长为352 nm，西红花苷I和西红花苷II检测波长为455 nm。结果 该方法分离度良好，标准曲线在线性范围内相关性良好，平均回收率为97.13%～101.42%。对5批10份坐珠达西测定和主成分分析（PCA），结果显示不同厂家的产品各聚一类，表明不同厂家产品质量差异较大；偏最小二乘法（PLS）的打分图分析表明，8种指标成分中没食子酸、苯甲酸、山柰酚、去氢木香烃内酯和西红花苷II对坐珠达西的质量贡献尤为明显。结论 该方法稳定可靠、结果准确，具有较好的稳定性和重复性，可用于坐珠达西的HPLC定量检测和质量控制。

Objective To establish an HPLC method for the simultaneous determination of eight components in Tibetan medicine Zuozhudaxi, and improve the standard of HPLC test. Methods The HPLC analysis was performed on a Kromasil C₁₈ column (250 mm ×4.6 mm, 5 μm). The column temperatrue was set at 30 ℃. A mixture of methyl alcohol-0.05% phosphoric acid aqueous solution was used as the mobile phase, gradient eluted with the flow rate at 1 mL/min, and detected by different UV wave lengths, i.e. 225 nm for gallic acid, benzoic acid, kaempferol, costunolide, and dehydroepiandrosterone costunolide, 352 nm for chlorogenic acid, and 445 nm for crocin I and crocin II. Results The eight components were well separated with ideal linear correlations. The average recoveries were in the range of 97.13%—101.42%. The contents of the eight components between two manufactures were different. PLS-DA loading plot of the samples showed that gallic acid, benzoic acid, kaempferol, dehydroepiandrosterone costunolide, and crocin II were the main components which made contributions to the quality of Zuozhudaxi. Conclusion The method is reliable, stable, accurate, and reproducible for the quantitative determination and quality control of Zuozhudaxi.

国家“十二五”科技支撑计划（2012BAI27B07）