目的 优选补骨脂-肉豆蔻药对醇提的最佳工艺条件。方法 通过L₉（3⁴） 正交试验设计，考察乙醇体积分数、乙醇用量、提取时间、提取次数对提取工艺的影响，以HPLC指纹图谱特征峰总面积与补骨脂素、异补骨脂素、去氢二异丁香酚的量以及干浸膏得率为综合评判指标。结果 最佳醇提工艺为乙醇体积分数50%，乙醇6倍用量，每次提取2 h，提取3次。结论 本法为补骨脂-肉豆蔻药对醇提工艺的确定提供了依据。

Objective To optimize the ethanol extraction process of Psoraleae Fructus-Myristicae Semen（psoralen-nutmeg）drug pair. Methods Using L₉(3⁴) orthogonal design, the effects of ethanol concentration, ethanol amount, extraction time, and extraction times on the extraction process were investigated. The contents of psoralen, isopsoralen, and dehydrodiisoeugenol, dry extract yield, and total area of HPLC fingerprint characteristic peaks were used as comprehensive evaluation indexes. Results The optimum process conditions were as follows：50% ethanol, six times of the ethanol volume, extracted for three times, each time for 2 h. Conclusion The method provides the basis for the determination of ethanol extraction process of Psoraleae Fructus-Myristicae Semen drug pair.

国家中医药重点学科临床中药学建设项目（国中医药人教发[2012] 32号）