目的 研究合子草Actinostemma lobatum全草的化学成分。方法 采用硅胶柱色谱、反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱等技术分离纯化，并结合理化性质和波谱学鉴定化合物的结构。结果 从合子草全草75%乙醇提取物中分离得到16个化合物，分别鉴定为合子草皂苷A（1）、合子草皂苷C（2）、合子草皂苷D（3）、合子草皂苷E（4）、葫芦素E（5）、β-谷甾醇（6）、胡萝卜苷（7）、α-菠菜甾醇（8）、α-菠菜甾醇-3-O-β-D-吡喃葡萄糖苷（9）、22，23-二氢-α-菠甾酮（10）、槲皮素（11）、山柰酚（12）、芦丁（13）、槲皮苷（14）、山柰酚-3-O-α-L-吡喃鼠李糖 （1→6）-β-D-吡喃葡萄糖苷（15）、异槲皮苷（16）。结论 化合物5～10为首次从该属植物中分离得到。

Objective To study the chemical constituents from the whole plant of Actinostemma lobatum. Methods The compounds were isolated and purified by various column chromatographies. Their structures were elucidated by means of chemical evidences and spectral analyses (MS, ¹H-NMR, and ¹³C-NMR). Results Sixteen compounds were isolated and identified as actinostemmoside A (1), actinostemmoside C (2), actinostemmoside D (3), actinostemmoside E (4), cucurbitacin E (5), β-sitosterol (6), daucosterol (7), α-spinasterol (8), α-spinasterol-3-O-β-D-glucopyranoside (9), 22, 23-dehydroxy-α-chondrillasterone (10), quercetin (11), kaempferol (12), rutin (13), quercitrin (14), kaempferol-3-O-α-L-rhamnopyranosyl (1→6)-β-D-glucoypyranoside (15), and isoquercitrin (16). Conclusion Compounds 5—10 are obtained from this genus for the first time.

江西省教育厅青年项目（GJJ14710）