目的 建立巴戟天Morinda officinalis不同炮制品蒽醌类成分指纹图谱，为巴戟天不同炮制品质量评价及炮制原理提供依据。方法 采用HPLC色谱法，色谱柱Ecosil ODS C₁₈柱（250 mm×4.6 mm，5 μm），流动相为甲醇-0.1%磷酸水溶液，梯度洗脱，柱温28 ℃，检测波长277 nm。对巴戟天4种炮制品进行指纹图谱研究，采用指纹图谱相似度软件进行分析。结果 建立了巴戟天不同炮制品的蒽醌类成分指纹图谱，分别指认了巴戟天、巴戟肉、盐巴戟5个色谱峰，制巴戟6个色谱峰。不同产地巴戟天质量差异显著。巴戟天不同炮制品间蒽醌类成分差异不大，但其量有变化，制巴戟由于炮制过程中使用了辅料甘草水溶液，新增了成分甘草素。结论 本法精密度、重复性、稳定性良好，利用HPLC指纹图谱分析方法，可较全面地反映巴戟天不同炮制品的差异。

Objective To establish an HPLC fingerprint of anthraquinone components and to provide the basis for quality standard and processing principle of processed Morinda officinalis (Morindae Officinalis Radix). Methods HPLC method was employed and Ecosil ODS C₁₈ column (250 mm × 4.6 mm, 5 μm) was used at temperature of 28 ℃. The mobile phase was methanol-0.1% phosphoric acid solution with gradient elution. The detection wavelength was 277 nm. The experimental data were analyzed by computer aided similarity evaluation software. Results HPLC fingerprint for anthraquinone components of different processed products of M. officinalis was established. Five chromatographic peaks of M. officinalis, morinda pulp, and salt-steamed M. officinalis and six chromatographic peaks of licorice-processed M. officinalis were respectively identified. The quality difference of M. officinalis among ten-origins reached significant level. The difference of anthraquinone components was not significant among the various processed products, but the content was changed. Liquiritigenin was found in licorice-processed M. officinalis. Conclusion The method is accurate, repeatable, and reliable, which can be used to identify different processed M. officinalis.

国家自然科学基金青年基金（81001635）；国家发改委行业专项（201107007）