目的 研究两面针药材提取物HPLC指纹图谱与抗肝癌活性之间的关系。方法 以人肝癌细胞株HepG2为药效模型，采用MTT法测定33批不同产地两面针药材提取物的抗肝癌活性，采用逐步后退法将量化特征峰面积与MTT法测得两面针提取物抗肿瘤活性的结果结合，构建两面针药材提取物抗肝癌的谱效关系方程。结果 建立的两面针药材提取物HPLC指纹图谱相似度达到0.7以上，经相似度软件筛选出24个特征指纹峰，用逐步后退法拟合两面针药材提取物抗肝癌谱效关系方程，该方程具有统计学意义和实际意义，可较准确地预测两面针药材提取物的抗肝癌活性。结论 两面针药材提取物抗肝癌活性是多种成分协同、互补、制约相互作用的结果，根据方程可预测肿瘤细胞的敏感性。

Objective To study the relationship between HPLC fingerprint and antihepatoma activity of the extract from Zanthoxyli Radix. Methods Zanthoxyli Radix fingerprint was established and human hepatic carcinoma cell line HepG2 was chosen as efficacy model. The antihepatoma activity of the extracts in Zanthoxyli Radix of 33 batchs from different habitats was determined by MTT method. After integrated the area of characteristic peak with the antihepatoma activity of Zanthoxyli Radix, the hepatic carcinoma “spectrum-effect” relationship was established by step-back technique. Results The HPLC-fingerprint of Zanthoxyli Radix was established, and the similarity of fingerprint had reached above 0.7. All data were done with the chromatographic fingerprint similarity evaluation system software, chosen out 24 fingerprint peaks. The spectrum-effect relationship on HepG2 was established and this relationship was significantly different. The results indicated that the multiple linear regression could predict the antihepatoma activity to HepG2 with statistical and practical significance. Conclusion The spectrum-effect relationship on HepG2 has the good predictive and describing abilities to evaluate the quality and therapeutic activity of Zanthoxyli Radix. According to the spectrum-effect relationship equation, we can predict the sensibility of different tumor cells.

国家自然科学基金地区科学基金项目（30760302）