目的 探讨绿原酸对光老化人皮肤成纤维细胞ESF-1光损伤的保护作用，及其对细胞外调节蛋白激酶（ERK）信号通路的影响。方法 用20 J/cm²的长波紫外线（UVA）照射ESF-1细胞制备光老化模型，以不同浓度绿原酸处理光老化细胞，检测细胞中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性、丙二醛（MDA）量，RT-PCR法检测细胞中ERK1、ERK2、基质金属蛋白酶1（MMP-1）及基质金属蛋白酶抑制因子1（TIMP-1） mRNA的表达量，Western blotting法检测p-ERK1/2蛋白的表达，酶联免疫法检测细胞上清液中MMP-1、TIMP-1的量。结果 UVA照射ESF-1细胞后，SOD活性、GSH-Px活性、TIMP-1 mRNA的表达量、TIMP-1的量明显降低，MDA水平、ERK1、ERK2、MMP-1 mRNA的表达量、p-ERK1/2、MMP-1水平明显升高（P＜0.01）；10.0、1.0 μmol/L绿原酸能够显著改善光老化ESF-1细胞的损伤（P＜0.05、0.01）。结论 绿原酸调控光老化ESF-1细胞ERK信号通路，从而减轻UVA对ESF-1细胞的损伤。

Objective To investigate the regulation of chlorogenic acid on ERK signal pathway and the protection of chlorogenic acid on photoaging ESF-1 cells. Methods The model of photoaging ESF-1 was established by irradiating of ultraviolet light (20 J/cm²). The chlorogenic acid at different concentration was used for photoaging cell. The SOD and GSH-Px activities, and MDA content were assayed by hydroxylamine, colorimetric and TBA method, respectively. The mRNA expression levels of ERK1, ERK2, MMP-1, and TIMP-1 were determined by RT-PCR, the protein expression of p-ERK1/2 was determined by Western blotting assay and the secretion levels of MMP-1 and TIMP-1 of cultured ESF-1 cells were detected by ELISA. Results After the UVA irradiation, the activities of SOD and GSH-Px, the mRNA expression level of TIMP-1, and the content of TIMP-1 were decreased, the contents of MDA, p-ERK1/2 and MMP-1, the mRNA expression levels of ERK1, ERK2, and MMP-1 were increased (P < 0.01). The above changes were inhibited by 10.0 and 1.0 μmol/L chlorogenic acid to improve the injury of ESF-1 cells (P < 0.05 , 0.01). Conclusion The chlorogenic acid could regulate ERK signal pathway and inhibit the injury of ESF-1 cells by UVA.

基金项目：国家自然科学基金资助项目（81274035）；黑龙江省青年科学基金资助项目（QC2010085）；黑龙江省中医药管理局科研基金项目（ZHY12-W012）；黑龙江中医药大学创新人才支持计划项目