目的 研究黄芪甲苷和三七的主要有效成分人参皂苷Rg₁、人参皂苷Rb₁、三七皂苷R₁分别配伍对小鼠脑缺血再灌注损伤后脑组织能量代谢的影响。方法 C57BL/6小鼠随机分组，连续给药3 d，末次给药1 h后，结扎双侧颈总动脉造成脑缺血20 min，再灌注24 h。采用HPLC法测定脑组织ATP、ADP、AMP水平，计算能荷（EC）值；采用逆转录聚合酶链式反应（RT-PCR）法和Western-blotting法测定脑组织葡萄糖转运蛋白3（GLUT3）基因和蛋白表达。结果 模型组脑组织ATP、ADP、AMP的量及EC值显著降低（P＜0.01），GLUT3基因和蛋白表达显著增强（P＜0.05）。黄芪甲苷、人参皂苷Rg₁、人参皂苷Rb₁、三七皂苷R₁、黄芪甲苷＋人参皂苷Rg₁、黄芪甲苷＋人参皂苷Rb₁、黄芪甲苷＋三七皂苷R₁均可显著增加脑组织ATP、ADP、AMP的量，增强脑组织GLUT3基因和蛋白表达，黄芪甲苷＋人参皂苷Rg₁、黄芪甲苷＋人参皂苷Rb₁和黄芪甲苷＋三七皂苷R₁改善上述指标的作用强于其单个有效成分；除4个有效成分单用外，黄芪甲苷＋人参皂苷Rg₁、黄芪甲苷＋人参皂苷Rb₁和黄芪甲苷＋三七皂苷R₁可显著增加EC值；黄芪甲苷＋人参皂苷Rg₁、黄芪甲苷＋人参皂苷Rb₁和黄芪甲苷＋三七皂苷R₁配伍对改善脑组织能量代谢指标具有协同或相加作用。结论 黄芪甲苷和三七的主要有效成分可改善脑缺血再灌注损伤后脑组织能量代谢，促进缺血脑组织对能量物质的利用，黄芪甲苷与人参皂苷Rg₁、人参皂苷Rb₁、三七皂苷R₁配伍对改善脑缺血后脑组织能量代谢具有增效作用。

Objective To investigate the effects of astragaloside IV respectively combined with the effective components of Panax notoginseng such as ginsenoside Rg₁, ginsenoside Rb₁, and notoginsenoside R₁ on energy metabolism of brain tissues after cerebralischemic-reperfusion in mice. Methods C57BL/6 mice were randomly grouped and treated for 3 d. After 1 h of the last administration, cerebral ischemia-reperfusion injury was established by bilateral common carotid artery (CCA) ligation for 20 min followed by reperfusion for 24 h. The contents of ATP, ADP, and AMP were determined by high performance liquid chromatography (HPLC) and the level of energy charge (EC) was calculated. The expression of glucose transporter 3 (GLUT3) gene and protein in brain tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blotting. Results In the model group, the contents of ATP, ADP, and AMP and the level of EC were significantly decreased (P < 0.01), and the expression of GLUT3 protein was significantly up-regulated in brain tissues (P < 0.05). Astragaloside IV, ginsenoside Rg₁, ginsenoside Rb₁, notoginsenoside R₁, astragaloside IV + ginsenoside Rg₁, astragaloside IV + ginsenoside Rb₁, and astragaloside IV + notoginsenoside R₁ could obviously increase the contents of ATP, ADP, and AMP and strengthen the expression of GLUT3 gene and protein. The effects of astragaloside IV + ginsenoside Rg₁, astragaloside IV + ginsenoside Rb₁, and astragaloside IV + notoginsenoside R₁ were better than those of the active components alone; Except four active components alone, EC level was obviously increased in astragaloside IV + ginsenoside Rg₁, astragaloside IV + ginsenoside Rb₁, and astragaloside IV + notoginsenoside R₁ groups; astragaloside IV respectively combined with ginsenoside Rg₁, ginsenoside Rb₁, and notoginsenoside R₁ had the synergistic or additive effects on improving the indexes of energy metabolism in brain tissues. Conclusion Astragaloside IV and the main active components of P. notoginseng could improve the energy metabolism in brain tissues after cerebral ischemic-reperfusion and promote the use of energy material in ischemic brain tissues, which is enchanced in astragaloside IV + ginsenoside Rg₁, astragaloside IV + ginsenoside Rb₁, and astragaloside IV + notoginsenoside R₁ groups.

国家自然科学基金资助项目（81102557）；教育部高等学校博士学科点专项科研基金资助项目（20104323110001）；湖南省高校创新平台开放基金资助项目（11K050）；湖南省中医药管理局重点项目（201301）；湖南省教育厅项目（11C0963）；“中西医结合防治心脑血管疾病的相关基础研究”湖南省高校科技创新团队；“中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金支持计划项目