目的 研究粒径对常用中药甘草粉末中活性成分（甘草酸、甘草苷）的质量分数及其体外溶出性能的影响，为甘草粉末的制备提供适宜的粒径范围。方法 将甘草粉碎、过筛制成7种不同粒径规格的样品并进行其粒度测试和扫描电镜分析，采用TCL法对样品进行定性鉴别；采用HPLC法分析样品中甘草酸、甘草苷的量及其体外溶出性能。结果 样品中甘草苷和甘草酸的量随粒径减小而增大。2种成分的量在300～900 μm、105～300 μm及小于105 μm这3种粒径间波动比较平稳，且粒径105～150 μm样品有效成分有良好的体外释放度。结论 在甘草粉末制备过程中，将粒径控制在105～150 μm，可使样品中活性成分甘草酸、甘草苷的量较高、稳定并有良好的体外释放性能。

Objective To study the effect of particle size on the contents and in vitro dissolution performances of the active ingredients (glycyrrhizic acid and liquiritin) in Glycyrrhizae Radix et Rhizoma powder, for the purpose of providing the appropriate particle size during the preparation of Glycyrrhizae Radix et Rhizoma powder. Methods Seven Glycyrrhizae Radix et Rhizoma powder samples with different particle sizes were prepared in present work. Their micro-topography structures were characterized by particle size distribution and scanning electron microscopy (SEM). Thin-layer chromatography (TLC) was applied to qualitatively identify these samples. The contents and in vitro release performance of glycyrrhizic acid and liquiritin in the seven powder samples were analyzed by high performance liquid chromatography (HPLC). Results The contents of glycyrrhizic acid and liquiritin increased with the decrease of particle sizes of the Glycyrrhizae Radix et Rhizoma powder samples. When the powder sizes were in the range of 300－900 μm, 105－300 μm or less than 105 μm, the contents of liquiritin and glycyrrhizic acid were stable. And 105－150 μm sample had good release performance in vitro. Conclusion Controlling the particle size between 105 and 150 μm during the granulation process of preparing Glycyrrhizae Radix et Rhizoma powder would make the contents of liquiritin and glycyrrhizic acid in Glycyrrhizae Radix et Rhizoma powder higher, stable and have good in vitro release performance.

上海市科学技术委员会支撑计划项目（13401901100）