目的 在非水相中利用酶法合成胆固醇癸二酸单烯酯，并对其最优合成工艺条件进行研究。方法 利用TLC、MS和NMR法对合成产物进行结构鉴定；通过正交试验对合成处方工艺进行优化。结果 最佳反应条件为胆固醇与癸二酸二乙烯酯物质的量之比为1∶6，Candida rugosa Lipase 10 mg/mL，异辛烷5 mL，温度35 ℃，反应时间24 h。结论 该条件下酯化率可达95%以上。

Objective To synthesize cholesteryl vinyl hemi-sebacate from cholesterol and divinyl sebacate in non aqueous phase. Methods TLC, MS, and NMR were used to identify the structure of production; the technological conditions of enzymatic esterification were determined through orthogonal test. Results The best condition: isooctane 5 mL, reaction temperature 35 ℃, reaction time 24 h, Candida rugosa Lipase 10 mg/mL, molar ratio of cholesterol to divinyl sebacate 1∶6. Conclusion The highest esterification rate is above 95%.

国家自然科学基金资助项目（30772790）