目的 对朝鲜白头翁Pulsatilla cernua根中非皂苷类成分进行研究。方法 采用开放硅胶柱色谱、凝胶柱色谱、循环制备高效液相色谱等进行分离纯化，通过核磁共振、质谱等波谱手段对化合物进行结构鉴定。结果 从朝鲜白头翁干燥根75%乙醇提取物中分离得到15个化合物，分别鉴定为5-羟基-2, 2-二甲基-苯并二氢吡喃-6-醛（1）、白头翁醌（2）、橙黄胡椒酰胺乙酸酯（3）、豆甾醇（4）、棕榈酸（5）、橙黄胡椒酰胺（6）、松脂素（7）、4″-甲氧基罗汉松脂素（8）、新海胆灵A（9）、(2S, 3S, 4R, 8E)-2-[(2′R)-2′-羟基十六烷酸酰胺]-8-十八烯-1, 3, 4-三醇（10）、5R-5-羟甲基-2(3H)-呋喃酮（11）、咖啡酸（12）、6-去羟基迷迭香酸甲酯（13）、常春藤酮酸（14）、5-羟基-6, 8-二甲氧基香豆素（15）。结论 化合物1为首次分离得到的天然产物，化合物3、6、8～10、13、15为首次从本属植物中分离得到，化合物7为首次从该植物中分离得到。

Objective To isolate and identify the chemical constituents from the roots of Pulsatilla cernua. Methods Compounds were isolated by various kinds of column chromatographies on silica gel, Sephadex LH-20, and recycling preparative HPLC, and their structures were elucidated by the physicochemical characters and spectral analysis. Results Fifteen chemical constituents were obtained and identified as 5-hydroxy-2, 2-dimethyl-2H-chromene-6-carbaldehyde (1), pulsaquinone (2), aurantiamide acetate (3), stigmasterol (4), hexadecanoic acid (5), aurantiamide (6), pinoresinol (7), buplerol (8), neoechinulin A (9), (2S, 3S, 4R, 8E)-2-[(2′R)-2′-hydroxypalmitoylamino]-8-octadecene-1, 3, 4-triol (10), 5R-dihydro-5-hydroxymethyl-2(3H)-furanone (11), caffeic acid (12), methyl 6-dehydroxyl rosmarinate (13), hederagonic acid (14), and 5-hydroxy-6, 8- dimethoxycoumarin (15), respectively. Conclusion Compound 1 is a new natural product, compounds 3, 6, 8—10, 13, and 15 are isolated from the plants in Pulsatilla Adans. for the first time, and compound 7 is isolated from this plant for the first time.