目的 分析腐胺和过氧化氢调控白桦三萜合成中是否存在交互作用。方法 在白桦悬浮细胞的生长末期添加1 mmol/L腐胺（Put）、0.1 mmol/L过氧化氢（H₂O₂）和40 μg/mL真菌诱导子，采用比色法、高效液相色谱法和荧光显微镜分析白桦悬浮细胞中三萜量、多胺量和H₂O₂荧光强度变化。结果 1 mmol/L Put增加了细胞中H₂O₂的荧光强度和三萜量。0.1 mmol/L H₂O₂促进了三萜合成，却抑制了多胺中亚精胺（Spd）、精胺（Spm）的合成，而对Put无影响。Put与H₂O₂同时处理组，细胞中H₂O₂的荧光强度和多胺的量均介于Put与H₂O₂单独处理组之间，而三萜量却显著高于其单独处理组；进一步药理学实验分析发现，真菌诱导子与H₂O₂清除剂过氧化氢酶（CAT）处理后，细胞中的Put和Spd量比真菌诱导后增加了0.62%和16.05%，而对Spm无显著影响。真菌诱导子与多胺（Pas）合成抑制剂D-精氨酸（D-arg）处理组，细胞中的H₂O₂荧光强度比真菌诱导子处理组有所降低了；在真菌诱导子、CAT和D-arg同时处理下，细胞中的H₂O₂荧光强度、PAs量和三萜量均低于真菌诱导子与CAT或D-arg处理，但三萜量仍高于对照组。结论 Put与H₂O₂在促进白桦三萜合成中存在交互作用。

Objective To analyze the interaction between putrescine (Put) and hydrogen peroxide (H₂O₂) on the regulation of triterpenoid production in Betula platyphylla suspension cell culture. Methods Put (1 mmol/L), H₂O₂ (0.1 mmol/L), and fungal elicitors (40 μg/mL) were added into the eight-day-old B. platyphylla suspension cell culture, and the changes of triterpenoid content, polyamine content, and H₂O₂ fluorescence intensity were analyzed by chemical colorimetry, HPLC, and fluorescence microscopy, respectively. Results Put (1 mmol/L) increased the H₂O₂ fluorescence intensity and triterpenoid content in B. platyphylla suspension cell culture. H₂O₂ (0.1 mmol/L) promoted the triterpenoid production, inhibited the production of spermidine (Spd) and spermine (Spm), and had no effectt on Put content. Under the treatment of 1 mmol/L Put and 0.1 mmol/L H₂O₂, H₂O₂ fluorescence intensity and polyamine (PAs) content were between its separated treatment, and triterpenoid content was significantly higher than its separated treatment. Comparing with the fungal elicitor, fungal elicitor and H₂O₂ scavenger catalase (CAT) induced an increase of 0.62% and 16.05% in Put and Spd content, respectively, while no effect on Spm content. Fungal elicitor and PAs synthesis inhibitor D-arginine (D-arg) decreased the H₂O₂ fluorescence intensity. Under the treatment of fungal elicitor, the fluorescence intensity of CAT and D-arg, H₂O₂ and the contents of PAs and triterpenoid were lower than those of fungal elicitor and CAT or D-arg, but triterpenoid content was still higher than that of the control. Conclusion This can be inferred that the interaction between Put and H₂O₂ regulates the triterpenoid production in birch suspension cell culture.

中央高效基本科研业务专项资金项目（DL13EA08-03）；国家自然科学基金资助项目（31100445，31070531）；黑龙江省博士后科研启动基金（LBH-Q11185）