目的 应用大孔吸附树脂结合聚酰胺柱色谱法富集分离独一味中黄酮类、苯乙醇苷类、环烯醚萜苷类及大极性成分（生物碱盐、糖类等），并实现工业化生产。方法 独一味水提物经聚酰胺和大孔吸附树脂串联及并联方法，用CCPP系列色谱设备进行分离富集，LM-125型超滤设备超滤纯化，JYT-50 LN多功能动态热回流提取浓缩机组减压干燥，分别富集得到水洗脱物、70%乙醇洗脱物I（70%乙醇洗脱聚酰胺柱）、70%乙醇洗脱物II（70%乙醇洗脱大孔吸附树脂柱）、超滤物；采用一阶导数紫外分光光度法测定总环烯醚萜苷的量，采用碱性硝酸铝-亚硝酸钠显色分光光光度法测定总黄酮和总苯乙醇苷的量，采用HPLC法测定山栀苷甲酯、8-O-乙酰山栀苷甲酯。结果 采用70%乙醇为溶媒，聚酰胺柱采用“上进下出”的进样方式和“上进下出”的洗脱方式，大孔吸附树脂柱采用“下进上出”的进样方式和“上进下出”的洗脱方式时，70%乙醇洗脱物I、70%乙醇洗脱物II、超滤物的得率分别为2.43%、20.80%、82.60%。结论 此生产工艺方法及参数可以成功地将实验成果转化为工业化生产。

Objective The macroporous adsorptive resin chromatographic column combining with polyamide chromatographic column was used to separate the flavonoides, phenylethanoidglycosides, iridoid glycosides, and high polarity ingredients (such as alkaloid salts and sugars) in industrialization. Methods Water extract from Lamiophlomis rotata (WELR) was separated and enriched by series and parallel method of polyamide and macroporous adsorptive resins and CCPP serial laminar analysis, and was ultrafiltrated by LM-125 ultrafiltration apparatus, then was dried under the reduced pressure by JYT-50 LN multipurpose kinesis back flow extract concentration subassembly. WELR, 70% ethanol solution eluate I (70% ethanol solution eluted polyamide column), 70% ethanol solution eluate II (70% ethanol solution eluting macroporous resin column), and ultrafiltration matter. The total flavonoids and total phenylethanoid glycosides were determined by aluminum nitrate-sodium nitrite UV spectrophotometry. The total iridoid glycosides were determined by the first derivative spectrophotometry. Shanzhiside methylester and 8-O-acetyl shanzhiside methylester were determined and the changes of total iridoid glycosides ingredients were investigated in the different elution requirement by HPLC. Results Ethanol (70%) was used as dissolvent. Using the “introduction from superior surface” sample introduction mode and “introduction from superior surface” eluting mode in polyamide chromatographic column, the “introduction from inferior surface” sample introduction mode and “introduction from superior surface” eluting mode in the macroporous adsorptive resin chromatographic column, yields of 70% ethanol solution eluate I, 70% ethanol solution eluate II, and ultrafiltration matter were 2.43%, 20.80%, and 82.60%, respectively. Conclusion This production technology may transform the experimental achievement to industrial production successfully.

国家科技部重大专项（2008ZXJ09004-XXX）；全军中医药研发推广项目（2006032001）；甘肃省科技重大专项（1102FKDA01国家自然科学基金资助项目（U1204323）；河南省高等学校青年骨干教师资助计划（2010GGJS-075）；河南省科技厅国际合作项目资助（134300510052）