目的 制备茶碱分子印迹聚合物微球，考察模板分子与交联剂的配比、反应时间、溶剂对分子印迹聚合物微球形貌、产率以及性能的影响。方法 以茶碱为模板分子，丙烯酰胺（AM）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，采用沉淀聚合法合成分子印迹微球，采用静态吸附、扫描电镜（SEM）对微球进行表征。结果 当模板分子与交联剂配比为1∶16、反应时间为24 h、乙腈为溶剂时，所制得印迹聚合物微球的形貌和吸附性能较好，对茶碱与咖啡碱的选择性分离因子α为1.74。结论 分子印迹聚合物微球对茶碱分子有特异性吸附和识别能力。

Objective To snythesize theophylline molecular imprinted polymer (MIP) microspheres. The influences of reaction conditions, including the category of solvent, reaction time, and the ratio of template to the cross-linking agent on the morphology and adsorption properties of MIP microspheres were studied. Methods Theophylline MIP microspheres were prepared by precipitation polymerization with theophylline as template and ethyleneglycol dimethacrylate (EGDMA) as cross-linker. The microspheres were characterized by static adsorption and scanning electron microscopy (SEM). Results The ratio of template to the cross-linking agent was 1∶16, reaction time was 24 h, and acetontrile was the solvent. The theophylline MIP microspheres were regular microspheres. The MIP microspheres showed the high adsorption capacity and the partition coefficient of the microspheres adsorbing theophylline was 1.74. Conclusion Theophylline MIP microspheres have the specific adsorption and recognition capabilities to the theophylline molecules.

国家自然科学基金资助项目（20976204）；广东省自然科学基金资助项目（s2012010009798）