目的 研究聚乙二醇-6000（polyethylene glycol-6000，PEG-6000）胁迫对丹参毛状根丹参酮类成分积累的影响。方法 用发根农杆菌ATCC15834诱导生产丹参毛状根，经20 d悬浮振荡培养后，分别采用1.2%、2.0%、5.5%、10%的PEG-6000处理，并用HPLC法测定PEG-6000处理1周后丹参毛状根中丹参酮I、隐丹参酮、二氢丹参酮I、丹参酮II_A的量。结果 PEG-6000能够抑制丹参毛状根的生长，PEG-6000为1.2%、2.0%、5.5%和10%处理条件下，丹参毛状根干质量分别减少为对照组的75.1%、83.0%、76.2%、76.1%；PEG-6000处理能够显著提高丹参毛状根中4种丹参酮的量，2.0%、5.5%的PEG-6000能够显著促进丹参酮I量的提高，1.2% PEG-6000能够显著提高隐丹参酮量的；二氢丹参酮I的量以2.0% PEG-6000处理条件下最高；丹参酮II_A的量以5.5% PEG-6000处理条件下最高。结论 PEG-6000能显著促进丹参毛状根中丹参酮类成分的积累。

Objective To study the effects of polyethylene glycol-6000 (PEG-6000) stress on the accumulation of tanshinones in hairy roots of Salvia miltiorrhiza. Methods Agrobacterium rhizogenes ATCC15834 was used to induce the hairy roots of S. miltiorrhiza. After 20 d suspension culture, the PEG-6000 (1.2%, 2.0%, 5.5%, and 10%, respectively) was added into the suspension cultures and at the same time, the contents of tanshinones (including tanshinone I, cryptotanshinone, dihydrotanshinon I, and tanshinone II_A) were quantified by HPLC on day 7. Results The growth of the hairy roots of S. miltiorrhiza was inhibited by PEG-6000. After PEG-6000 (1.2%, 2.0%, 5.5%, and 10%) treatment, the dry weights of the hairy roots of S. miltiorrhiza were reduced to 75.1%, 83.0%, 76.2%, and 76.1% of the control group, respectively. Addition of PEG-6000 at different levels could significantly increase the yields of four tanshinones in the hairy roots of S. miltiorrhiza. The yields of tanshinone I, cryptotanshinone, dihydrotanshinon I, and tanshinone II_A were significantly increased by 2.0%—5.5%, 1.2%, 2.0%, and 5.5% PEG-6000, respectively. And the tanshinone II_A increased most. Conclusion PEG-6000 could stimulate the accumulation of tanshinones in the hairy roots of S. miltiorrhiza.