目的 研究黄花香茶菜Isodon sculponeatus地下部分的化学成分及其生物活性。方法 采用70%丙酮常温提取，硅胶、葡聚糖凝胶和反相色谱法分离纯化，利用现代波谱技术进行结构鉴定；采用MTT法对70%丙酮提取物、醋酸乙酯部位、正丁醇部位等进行细胞毒活性活性测试。结果 从黄花香茶菜地下部分醋酸乙酯部位中分离得到14个化合物，分别鉴定为macrophynin E（1）、19-hydroxyferruginol（2）、2α, 3α, 24-trihydroxyursa-12, 20(30)-dien-28-oic acid（3）、山香三萜二烯酸（4）、stigmast-4-en-6β-ol-3-one（5）、豆甾-3β, 6α-二醇（6）、松柏醛（7）、邻苯二甲酸二异丁酯（8）、双 (2-乙基己基) 邻苯二甲酸酯（9）、正十六烷酸（10）、3β-羟基-5α, 8α-桥二氧麦角甾-6, 22E-二烯（11）、胡萝卜苷（12）、β-谷甾醇（13）、熊果酸（14）。活性研究显示，70%丙酮提取物、醋酸乙酯部位对肝癌细胞SMMC-7721有一定的抑制活性。结论 化合物1～10为首次从该种植物中分离得到，化合物2、3、5、6、8、9为首次从该属植物中分离得到。

Objective To study the chemical constituents from the underground parts from Isodon sculponeatus and their bioactivities. Methods The compounds were isolated with 70% acetone by chromatography with silica gel, RP-18, and Sephadex LH-20 columns. The obtained parts were identified by means of spectroscopic methods. The cytotoxic activities of the samples including 70% acetone extract, ethyl acetate (EtOAc) fraction, and butanol fraction were evaluated by MTT method. Results From the EtOAc fraction in the underground parts of I. sculponeata, fourteen compounds were isolated and identified as macrophynin E (1), 19-hydroxyferruginol (2), 2α, 3α, 24-trihydroxyursa-12, 20(30)-dien-28-oic acid (3), hyptadienic acid (4), stigmast-4-en-6β-ol-3-one (5), stigmastane-3β, 6α-diol (6), coniferyl aldehyde (7), 1, 2-benzenedicarboxylic acid bis (2-methylpropyl) ester (8), bis (2-ethylhexyl) phthalate (9), n-hexadecanioc acid (10), 5α, 8α-ergosta-6, 22E-dien-3β-ol (11), daucosterol (12), β-sitosterol (13), and ursolic acid (14). Results The bioassays indicated that both the 70% acetone extract and EtOAc fraction displayed the moderate cytotoxicities against the liver cancer cell line SMMC-7721. Conclusion All the compounds are isolated from the underground parts of I. sculponeata for the first time while compounds 1—10 are firstly isolated from this plant, and compounds 2, 3, 5, 6, 8, and 9 are isolated from the plants of Isodon (Hara.) for the first time.

国家自然科学基金资助项目（81060259）；云南省中青年学术技术带头人后备人才项目（2008PY005）；云南省首批引进海外高层次人才项目（云组通 [2011] 156号）